• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ADH1C Recombinant antibody

ADH1C Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241623C3

Cat no : 84546-1-RR

Synonyms

EC:1.1.1.1, ADH3, 241623C3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ADH1C Recombinant antibody (84546-1-RR)

    WB analysis using 84546-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84546-1-RR (ADH1C antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse liver tissue, mouse colon tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84546-1-RR is ADH1C Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ADH1C fusion protein Ag13528
Full Name alcohol dehydrogenase 1C (class I), gamma polypeptide
Calculated Molecular Weight 375 aa, 40 kDa
GenBank Accession NumberBC062476
Gene Symbol ADH1C
Gene ID (NCBI) 126
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Alcohol dehydrogenase 1C(ADH1C), also known as ADH3, is an alcohol dehydrogenase that can catalyze ethanol oxidation to metabolite acetaldehyde. According to THE HUMAN PROTEIN ATLAS database, ADH1C locates mainly to the cytosol and plasma membrane. Genetic polymorphism of the ADH1C gene (ADH1C*1 allele) is associated with various human cancers, such as gastric cancer, oral cancer and CRC, for it encodes an alcohol dehydrogenase producing 2.5 times more acetaldehyde.(PMID: 35300114)

Protocols

Product Specific Protocols
WB protocol for ADH1C antibody 84546-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols