ADH1C Polyclonal antibody

ADH1C Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 18897-1-AP

Synonyms

EC:1.1.1.1, ADH3

---

Validation Data Gallery

• 

  WB analysis using 18897-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18897-1-AP (ADH1C antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 18897-1-AP

  IP result of anti-ADH1C (IP:18897-1-AP, 4ug; Detection:18897-1-AP 1:1000) with mouse liver tissue lysate 4000 ug.

• 

  IHC staining of rat liver using 18897-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 18897-1-AP (ADH1C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 18897-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18897-1-AP (ADH1C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 18897-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18897-1-AP (ADH1C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 18897-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18897-1-AP (ADH1C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 18897-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18897-1-AP (ADH1C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18897-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18897-1-AP (ADH1C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 18897-1-AP

  Immunofluorescent analysis of HepG2 cells, using ADH1C antibody 18897-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of HepG2 using 18897-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ADH1C antibody (18897-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

---

Tested Applications

• Positive WB detected in: rat liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: rat liver tissue, human lung cancer tissue, human colon tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

18897-1-AP targets ADH1C in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADH1C fusion protein Ag13528
• Full Name: alcohol dehydrogenase 1C (class I), gamma polypeptide
• Calculated Molecular Weight: 375 aa, 40 kDa
• Observed Molecular Weight: 37-45 kDa
• GenBank Accession Number: BC062476
• Gene Symbol: ADH1C
• Gene ID (NCBI): 126
• RRID: AB_10596950
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADH1C(Alcohol dehydrogenase 1C) is also named as ADH3 and belongs to the zinc-containing alcohol dehydrogenase family.It catalyzes the rate-limiting step for ethanol metabolism: the oxidation of alcohol to acetaldehyde.The ADH1C gene is active in intestine and kidney in fetal and early postnatal life, and persists in the stomach and liver in adult life(PMID:4748759).

Publications

Species	Application	Title
mouse	WB	Free Radic Biol Med; PPARα agonist WY-14,643 enhances ethanol metabolism in mice: role of catalase.; Authors - Xue Chen
human,mouse	WB	Biochem Pharmacol; The role of alcohol dehydrogenase 1C in regulating inflammatory responses in ulcerative colitis.; Authors - Feifeng Song
mouse	WB	Biochem Pharmacol; PPARα agonist WY-14,643 induces adipose atrophy and fails to blunt chronic ethanol-induced hepatic fat accumulation in mice lacking adipose FGFR1.; Authors - Yunhui Xu