ADCY3 Polyclonal antibody

ADCY3 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 19492-1-AP

Synonyms

AC III, AC3, ADCY3, adenylate cyclase 3, Adenylate cyclase type 3, Adenylate cyclase type III, Adenylyl cyclase 3, ATP pyrophosphate lyase 3, KIAA0511

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Validation Data Gallery

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  WB analysis of mouse skeletal muscle using 19492-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19492-1-AP (ADCY3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 19492-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19492-1-AP (ADCY3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of MDCK using 19492-1-AP

  IP Result of anti-ADCY3 (IP:19492-1-AP, 4ug; Detection:19492-1-AP 1:500) with MDCK cells lysate 3500ug.

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  IHC staining of human kidney using 19492-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19492-1-AP (ADCY3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 19492-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19492-1-AP (ADCY3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 19492-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19492-1-AP (ADCY3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 19492-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19492-1-AP (ADCY3 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of MDCK using 19492-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using 19492-1-AP (ADCY3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of Mouse embryonic fibroblasts using 19492-1-AP

  IF result from Dr. Corbit, Kevin. anti-ADCY3 (19492-1-AP) mark the cilium of Mouse embryonic fibroblasts.

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  IF Staining of MDCK using 19492-1-AP

  Immunofluorescent analysis of MDCK cells using 19492-1-AP (ADCY3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, human brain tissue
• Positive IP detected in: MDCK cells
• Positive IHC detected in: human kidney tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MDCK cells,

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 7 publications below

Product Information

19492-1-AP targets ADCY3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: adenylate cyclase 3
• Calculated molecular weight: 129 kDa
• Observed molecular weight: 170-180 kDa
• GenBank accession number: NM_004036
• Gene symbol: ADCY3
• Gene ID (NCBI): 109
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ADCY3 is an adenylate cyclase (AC) functioning to convert ATP to cAMP in response to signals initiated by activation of Gs-coupled receptors. To date, 10 isoforms of AC have been cloned in mammals. Initially identified in olfactory cilia, ADCY3 is regarded as the olfactory isoform and required for detection of odorants. Recently it has been found that outside of the olfactory epithelium the olfactory-like signaling pathway including AC3 may also have a key role in spermatogenesis and spermatozoa functions. In addition, the ciliary location of AC3 makes it as a prominent marker for primary cilia in brain.AC3 is typically found at 130 kDa in olfactory tissues.In nonolfactory tissues (such as sperm), this protein is reported to migrate with a molecular mass of 55 kDa,occasionally accompanied by additional bands at either 90 or 130 kDa(PMID:9539154).

Publications

Species	Application	Title
rat	IF	STAR Protoc; A Cell-Based Assay Optimized for High-Content Cilia Imaging with Primary Rat Hippocampal Neurons.; Authors - Alessia Di Nardo
human	IF	Cell Rep; Phenotypic Screen with TSC-Deficient Neurons Reveals Heat-Shock Machinery as a Druggable Pathway for mTORC1 and Reduced Cilia.; Authors - Alessia Di Nardo
mouse	IF	Adv Sci (Weinh); Targeting the HDAC6-Cilium Axis Ameliorates the Pathological Changes Associated with Retinopathy of Prematurity.; Authors - Jie Ran
mouse	WB	Neurosci Res; Phenotypic characterization of Cdkl5-knockdown neurons establishes elongated cilia as a functional assay for CDKL5 Deficiency Disorder.; Authors - Alessia Di Nardo
human	IF	Phytochemistry; Hypoglycemic flavonoids from Selaginella tamariscina (P.Beauv.) Spring.; Authors - Hong-Ping Long
mouse	IF	Dev Dyn; Phosphosite T674A mutation in kinesin family member 3A fails to reproduce tissue and ciliary defects characteristic of CILK1 loss of function.; Authors - Casey D Gailey