ADCY3 Polyclonal antibody
ADCY3 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 19492-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse skeletal muscle tissue, human brain tissue |
Positive IP detected in | MDCK cells |
Positive IHC detected in | human kidney tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MDCK cells, |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 2 publications below |
IHC | See 1 publications below |
IF | See 7 publications below |
Product Information
19492-1-AP targets ADCY3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | adenylate cyclase 3 |
Calculated molecular weight | 129 kDa |
Observed molecular weight | 170-180 kDa |
GenBank accession number | NM_004036 |
Gene symbol | ADCY3 |
Gene ID (NCBI) | 109 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ADCY3 is an adenylate cyclase (AC) functioning to convert ATP to cAMP in response to signals initiated by activation of Gs-coupled receptors. To date, 10 isoforms of AC have been cloned in mammals. Initially identified in olfactory cilia, ADCY3 is regarded as the olfactory isoform and required for detection of odorants. Recently it has been found that outside of the olfactory epithelium the olfactory-like signaling pathway including AC3 may also have a key role in spermatogenesis and spermatozoa functions. In addition, the ciliary location of AC3 makes it as a prominent marker for primary cilia in brain.AC3 is typically found at 130 kDa in olfactory tissues.In nonolfactory tissues (such as sperm), this protein is reported to migrate with a molecular mass of 55 kDa,occasionally accompanied by additional bands at either 90 or 130 kDa(PMID:9539154).
Protocols
Product Specific Protocols | |
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WB protocol for ADCY3 antibody 19492-1-AP | Download protocol |
IHC protocol for ADCY3 antibody 19492-1-AP | Download protocol |
IF protocol for ADCY3 antibody 19492-1-AP | Download protocol |
IP protocol for ADCY3 antibody 19492-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
STAR Protoc A Cell-Based Assay Optimized for High-Content Cilia Imaging with Primary Rat Hippocampal Neurons. | ||
Cell Rep Phenotypic Screen with TSC-Deficient Neurons Reveals Heat-Shock Machinery as a Druggable Pathway for mTORC1 and Reduced Cilia. | ||
Adv Sci (Weinh) Targeting the HDAC6-Cilium Axis Ameliorates the Pathological Changes Associated with Retinopathy of Prematurity. | ||
Neurosci Res Phenotypic characterization of Cdkl5-knockdown neurons establishes elongated cilia as a functional assay for CDKL5 Deficiency Disorder. | ||
Dev Dyn Phosphosite T674A mutation in kinesin family member 3A fails to reproduce tissue and ciliary defects characteristic of CILK1 loss of function. |