ADCY3 Polyclonal antibody

ADCY3 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 19492-1-AP

Synonyms

AC3, AC III, ATP pyrophosphate-lyase 3, ADCY 3, AC-III

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Validation Data Gallery

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  WB analysis of mouse skeletal muscle using 19492-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19492-1-AP (ADCY3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 19492-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19492-1-AP (ADCY3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of MDCK using 19492-1-AP

  IP result of anti-ADCY3 (IP:19492-1-AP, 4ug; Detection:19492-1-AP 1:500) with MDCK cells lysate 3500ug.

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  IHC staining of human kidney using 19492-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19492-1-AP (ADCY3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 19492-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19492-1-AP (ADCY3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 19492-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19492-1-AP (ADCY3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 19492-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19492-1-AP (ADCY3 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of rat brain using 19492-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using ADCY3 antibody (19492-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat brain using 19492-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using ADCY3 antibody (19492-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MDCK using 19492-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using 19492-1-AP (ADCY3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of hTERT-RPE1 using 19492-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using ADCY3 antibody (19492-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  IF Staining of Mouse embryonic fibroblasts using 19492-1-AP

  IF result from Dr. Corbit, Kevin. anti-ADCY3 (19492-1-AP) mark the cilium of Mouse embryonic fibroblasts.

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, human brain tissue
• Positive IP detected in: MDCK cells
• Positive IHC detected in: human kidney tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: rat brain tissue
• Positive IF/ICC detected in: hTERT-RPE1 cells, MDCK cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 8 publications below

Product Information

19492-1-AP targets ADCY3 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

• Tested Reactivity: human, mouse, rat, canine
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: adenylate cyclase 3
• Calculated Molecular Weight: 129 kDa
• Observed Molecular Weight: 170-180 kDa
• GenBank Accession Number: NM_004036
• Gene Symbol: ADCY3
• Gene ID (NCBI): 109
• RRID: AB_10638445
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADCY3 is an adenylate cyclase (AC) functioning to convert ATP to cAMP in response to signals initiated by activation of Gs-coupled receptors. To date, 10 isoforms of AC have been cloned in mammals. Initially identified in olfactory cilia, ADCY3 is regarded as the olfactory isoform and required for detection of odorants. Recently it has been found that outside of the olfactory epithelium the olfactory-like signaling pathway including AC3 may also have a key role in spermatogenesis and spermatozoa functions. In addition, the ciliary location of AC3 makes it as a prominent marker for primary cilia in brain.AC3 is typically found at 130 kDa in olfactory tissues.In nonolfactory tissues (such as sperm), this protein is reported to migrate with a molecular mass of 55 kDa,occasionally accompanied by additional bands at either 90 or 130 kDa(PMID:9539154).

Publications

Species	Application	Title
mouse	IF	J Cell Biol; Sonic Hedgehog activates prostaglandin signaling to stabilize primary cilium length; Authors - Shariq S Ansari
mouse	IHC	Acta Physiol (Oxf); Aromatherapy: Activating Olfactory Calcium-sensing Receptors Impairs Renal Hemodynamics via Sympathetic Nerve-mediated Vasoconstriction.; Authors - Shih-Pin Lee
mouse	WB,IF	Hum Mol Genet; Shortened primary cilium length and dysregulated Sonic hedgehog signaling in Niemann-Pick C1 disease.; Authors - Sonia Canterini
rat	IF	Biomolecules; Preferential Expression of Ca2+-Stimulable Adenylyl Cyclase III in the Supraventricular Area, including Arrhythmogenic Pulmonary Vein of the Rat Heart.; Authors - Yosuke Okamoto
	WB,IHC	Front Physiol; Expression Regulation of Water Reabsorption Genes and Transcription Factors in the Kidneys of Lepus yarkandensis.; Authors - Shengjie Luo
mouse	IF	Dev Dyn; Phosphosite T674A mutation in kinesin family member 3A fails to reproduce tissue and ciliary defects characteristic of CILK1 loss of function.; Authors - Casey D Gailey