- Featured Product
- KD/KO Validated
ADARB1 Polyclonal antibody
ADARB1 Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse
Applications
WB, IP, IF, RIP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 22248-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, 4T1 cells, C2C12 cells, HeLa cells, NIH/3T3 cells, HepG2 cells |
Positive IP detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 3 publications below |
WB | See 11 publications below |
IHC | See 3 publications below |
IF | See 1 publications below |
RIP | See 1 publications below |
Product Information
22248-1-AP targets ADARB1 in WB, IP, IF, RIP, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.
Tested Reactivity | human, rat, mouse |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ADARB1 fusion protein Ag17743 |
Full Name | adenosine deaminase, RNA-specific, B1 (RED1 homolog rat) |
Calculated Molecular Weight | 741 aa, 81 kDa |
Observed Molecular Weight | 74-86 kDa |
GenBank Accession Number | BC065545 |
Gene Symbol | ADARB1 |
Gene ID (NCBI) | 104 |
RRID | AB_2879047 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ADARB1(Double-stranded RNA-specific editase 1) is also named as ADAR2, DRADA2, RED1 and belongs to the ADAR family. It catalyses the deamination of adenosine to inosine at the GluR2 Q/R site in the pre-mRNA encoding the critical subunit of AMPA receptors. This protein has some isoforms with the molecular weight from 77 kDa to 81 kDa and 7 kDa, but it can be detected a very strong band at 50 kDa in addition to a predicted band at 75 kDa as the result of western blot, while ADARB1 has several alternative splice variants (PMID:23103828).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for ADARB1 antibody 22248-1-AP | Download protocol |
IP protocol for ADARB1 antibody 22248-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Basic Res Cardiol Reduction of A-to-I RNA editing in the failing human heart regulates formation of circular RNAs. | ||
Sci Rep Functional interaction between FUS and SMN underlies SMA-like splicing changes in wild-type hFUS mice. | ||
FASEB J Lung fibrosis is induced in ADAR2 overexpressing mice via HuR-induced CTGF signaling.
| ||
J Cell Mol Med Lipopolysaccharide enhances ADAR2 which drives Hirschsprung's disease by impairing miR-142-3p biogenesis. | ||
Front Pharmacol Aberrant Expression of ADARB1 Facilitates Temozolomide Chemoresistance and Immune Infiltration in Glioblastoma. |