ADARB1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ADARB1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IP, IF, RIP, IHC, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 22248-1-AP

Synonyms

ADAR2, ADAR2a, ADAR2a L1, ADAR2a L2, ADAR2a L3, ADAR2b, ADAR2c, ADAR2d, ADAR2g, ADARB1, DRABA2, DRADA2, dsRNA adenosine deaminase, RED1, RNA editing deaminase 1, RNA editing enzyme 1

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Validation Data Gallery

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  WB analysis using 22248-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22248-1-AP (ADARB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22248-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22248-1-AP (ADARB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 22248-1-AP

  IP result of anti-ADARB1 (IP:22248-1-AP, 4ug; Detection:22248-1-AP 1:1000) with HeLa cells lysate 1080 ug.

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Tested Applications

• Positive WB detected in: A431 cells, 4T1 cells, C2C12 cells, HeLa cells, NIH/3T3 cells, HepG2 cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• RIP: See 1 publications below

Product Information

22248-1-AP targets ADARB1 in WB, IP, IF, RIP, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADARB1 fusion protein Ag17743
• Full Name: adenosine deaminase, RNA-specific, B1 (RED1 homolog rat)
• Calculated Molecular Weight: 741 aa, 81 kDa
• Observed Molecular Weight: 74-86 kDa
• GenBank Accession Number: BC065545
• Gene Symbol: ADARB1
• Gene ID (NCBI): 104
• RRID: AB_2879047
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADARB1(Double-stranded RNA-specific editase 1) is also named as ADAR2, DRADA2, RED1 and belongs to the ADAR family. It catalyses the deamination of adenosine to inosine at the GluR2 Q/R site in the pre-mRNA encoding the critical subunit of AMPA receptors. This protein has some isoforms with the molecular weight from 77 kDa to 81 kDa and 7 kDa, but it can be detected a very strong band at 50 kDa in addition to a predicted band at 75 kDa as the result of western blot, while ADARB1 has several alternative splice variants (PMID:23103828).

Publications

Species	Application	Title
human	WB	Nature; Breakage of cytoplasmic chromosomes by pathological DNA base excision repair.; Authors - Shangming Tang
human	WB,RIP	Basic Res Cardiol; Reduction of A-to-I RNA editing in the failing human heart regulates formation of circular RNAs.; Authors - Karoline E Kokot
mouse	IF	Sci Rep; Functional interaction between FUS and SMN underlies SMA-like splicing changes in wild-type hFUS mice.; Authors - Alessia Mirra
mouse	WB	FASEB J; Lung fibrosis is induced in ADAR2 overexpressing mice via HuR-induced CTGF signaling.; Authors - Ramani Soundararajan ; KO Validated
human	WB	J Cell Mol Med; Lipopolysaccharide enhances ADAR2 which drives Hirschsprung's disease by impairing miR-142-3p biogenesis.; Authors - Lei Peng
human	WB	Front Pharmacol; Aberrant Expression of ADARB1 Facilitates Temozolomide Chemoresistance and Immune Infiltration in Glioblastoma.; Authors - Can Lu