ADAMTS7 Polyclonal antibody

ADAMTS7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 26836-1-AP

Synonyms

ADAM TS 7, ADAM TS7, ADAMTS 7, ADAMTS7, COMPase

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Validation Data Gallery

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  WB analysis using 26836-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26836-1-AP (ADAMTS7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 26836-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26836-1-AP (ADAMTS7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 26836-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26836-1-AP (ADAMTS7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 26836-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26836-1-AP (ADAMTS7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 26836-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26836-1-AP (ADAMTS7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 26836-1-AP is ADAMTS7 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADAMTS7 fusion protein Ag25218
• Full Name: ADAM metallopeptidase with thrombospondin type 1 motif, 7
• Calculated Molecular Weight: 1686 aa, 184 kDa
• Observed Molecular Weight: 184 kDa
• GenBank Accession Number: BC061631
• Gene Symbol: ADAMTS7
• Gene ID (NCBI): 11173
• RRID: AB_2880653
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC,IF	Eur J Med Res; Diagnostic value of serum COMP and ADAMTS7 for intervertebral disc degeneration; Authors - Jing-Yu Ding