ADAMTS2 Polyclonal antibody

• 

  WB analysis using 31032-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31032-1-AP (ADAMTS2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 31032-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31032-1-AP (ADAMTS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 31032-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31032-1-AP (ADAMTS2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ADAMTS2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 31032-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

ADAMTS-2, pNPI, PCPNI, PCINP, ADAMTS 2

---

Tested Applications

• Positive WB detected in: A431 cells, A375 cells, PC-3 cells, THP-1 cell, COLO 205 cells
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31032-1-AP targets ADAMTS2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADAMTS2 fusion protein Ag34957
• Full Name: ADAM metallopeptidase with thrombospondin type 1 motif, 2
• Calculated Molecular Weight: 135 kDa
• Observed Molecular Weight: 110~130 kDa
• GenBank Accession Number: NM_014244
• Gene Symbol: ADAMTS2
• Gene ID (NCBI): 9509
• RRID: AB_3669823
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: O95450
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

A disintegrin and metalloproteinase with thrombospondin motifs 2 (ADAMTS-2) is a metalloproteinase that plays a key role in the processing of fibrillar procollagen precursors into mature collagen molecules by excising the amino-propeptide. ADAMTS-2 can, like the procollagen C-proteinases, be regulated by transforming growth factor-β1 (TGF-β1), with implications for mechanisms whereby this growth factor effects net increases in formation of extracellular matrix. Mutations in the ADAMTS-2 gene cause dermatosparaxis in human (dermatosparactic type of Ehlers-Danlos syndrome, also known as Ehlers-Danlos syndrome type VIIC). Human ADAMTS-2 has eight potential sites for Asn-linked glycosylation and other mammals (PMID: 16046392, 12646579).