ADAMTS14 Polyclonal antibody

ADAMTS14 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31075-1-AP

Synonyms

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Validation Data Gallery

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  WB analysis using 31075-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31075-1-AP (ADAMTS14 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 31075-1-AP

  K-562 cells lysates were subjected to SDS PAGE followed by western blot with 31075-1-AP (ADAMTS14 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HL-60 cells, K-562 cells, HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31075-1-AP targets ADAMTS14 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADAMTS14 fusion protein Ag34638
• Full Name: ADAM metallopeptidase with thrombospondin type 1 motif, 14
• Calculated Molecular Weight: 134 kDa
• Observed Molecular Weight: 134 kDa
• GenBank Accession Number: NM_080722
• Gene Symbol: ADAMTS14
• Gene ID (NCBI): 140766
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The ADAMTS (a disintegrin-like and metalloproteinase with thrombospondin motifs) is a family of extracellular metalloproteinases mediating diverse functions including matrix degradation, blood coagulation, and angiogenesis. The ADAMTS family constitutes a group of proteins composed of 19 enzymes and 7 ADAMTS-like proteins. ADAMTS14 possesses high similarity with ADAMTS2 and ADAMTS3 in sequence and domain composition (PMID: 11741898). The expression of ADAMTS14 is correlated with the occurrence and progression of colorectal cancer and oral squamous cell carcinoma (PMID: 34856162; PMID: 32102222).