ADAMTS12 Polyclonal antibody

• Featured Product
• KD/KO Validated

ADAMTS12 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 24934-1-AP

Synonyms

ADAM TS 12, ADAM TS12, ADAMTS 12, ADAMTS12, PRO4389

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Validation Data Gallery

• 

  WB analysis of mouse placenta using 24934-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 24934-1-AP (ADAMTS12 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 24934-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24934-1-AP (ADAMTS12 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of NIH/3T3 using 24934-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using ADAMTS12 antibody (24934-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: mouse placenta tissue, NIH/3T3 cells
• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 9 publications below
• IF: See 1 publications below

Product Information

24934-1-AP targets ADAMTS12 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADAMTS12 fusion protein Ag20712
• Full Name: ADAM metallopeptidase with thrombospondin type 1 motif, 12
• Calculated Molecular Weight: 178 kDa
• Observed Molecular Weight: 155 kDa
• GenBank Accession Number: BC058841
• Gene Symbol: ADAMTS12
• Gene ID (NCBI): 81792
• RRID: AB_2879806
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADAMTS12(A disintegrin and metalloproteinase with thrombospondin motifs 12) is also named as AT12, ADAM-TS12, PRO4389 and belongs to the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. It may play roles in pulmonary cells during fetal development or in tumor processes through its proteolytic activity or as a molecule potentially involved in regulation of cell adhesion. ADAMTS7 and ADAMTS12 appear to be potent negative regulators of endplate cartilage development(PMID:22247065). This protein has 3 isoforms produced by alternative splicing. The full length protein has 14 glycosylation sites.

Publications

Species	Application	Title
human	WB	Oncotarget; Cleavage of Fibulin-2 by the aggrecanases ADAMTS-4 and ADAMTS-5 contributes to the tumorigenic potential of breast cancer cells.; Authors - Tania Fontanil
human	WB,IF	Hum Mol Genet; Epigenetic changes in reterm birth placenta suggest a role for ADAMTS genes in spontaneous preterm birth.; Authors - Sneha Mani ; KD Validated
human	WB	Cell Signal; ADAMTS12 promotes oxaliplatin chemoresistance and angiogenesis in gastric cancer through VEGF upregulation; Authors - Yingming Jiang
human	WB	Sci Rep; ADAMTS12 acts as a tumor microenvironment related cancer promoter in gastric cancer.; Authors - Yangming Hou ; KD Validated
human	WB	Int J Mol Med; Overexpression of PRDM13 inhibits glioma cells via Rho and GTP enzyme activation protein.; Authors - Linna Zhang
human	WB	Hepatobiliary Pancreat Dis Int; ADAMTS12 promotes migration and epithelial-mesenchymal transition and predicts poor prognosis for pancreatic cancer.; Authors - Rui-Zhe He ; KD Validated