ADAM5 Polyclonal antibody, PBS Only

ADAM5 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 22147-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HeLa using 22147-1-AP (same clone as 22147-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 22147-1-AP (ADAM5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22147-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 22147-1-AP (same clone as 22147-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 22147-1-AP (ADAM5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22147-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 22147-1-AP (same clone as 22147-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 22147-1-AP (ADAM5 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22147-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 22147-1-AP (same clone as 22147-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 22147-1-AP (ADAM5 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22147-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 22147-1-AP (same clone as 22147-1-PBS)

  Immunofluorescent analysis of HeLa cells using 22147-1-AP (ADAM5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22147-1-PBS in a different storage buffer formulation.

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Product Information

22147-1-PBS targets ADAM5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADAM5 fusion protein Ag17590
• Full Name: ADAM metallopeptidase domain 5 pseudogene
• Calculated Molecular Weight: 412 aa, 47 kDa
• Observed Molecular Weight: 36-50 kDa
• GenBank Accession Number: BC067864
• Gene Symbol: ADAM5/ADAM5P
• Gene ID (NCBI): 255926
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ADAM5(putative disintegrin and metalloproteinase domain-containing protein 5) is also named as ADAM5P, TMDC2(tMDCII). The tMDC II, abundantly expressed, sperm surface MDC proteins identified in rodent and macaque systems, is also nonfunctional in the human(PMID:10417343). It has 7 isoforms produced by alternative splicing. It is expressed in testis highly by northern blot. This antibody is specific to ADAM5.