ADAM28 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

ADAM28 Polyclonal Antibody for WB, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 22234-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of mouse lung using 22234-1-AP (same clone as 22234-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 22234-1-AP (ADAM28 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22234-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse spleen using 22234-1-AP (same clone as 22234-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 22234-1-AP (ADAM28 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22234-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 22234-1-AP (same clone as 22234-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 22234-1-AP (ADAM28 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22234-1-PBS in a different storage buffer formulation.

• 

  WB analysis of BxPC-3 using 22234-1-AP (same clone as 22234-1-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 22234-1-AP (ADAM28 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22234-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 22234-1-AP (same clone as 22234-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 22234-1-AP (ADAM28 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22234-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-13 using 22234-1-AP (same clone as 22234-1-PBS)

  PC-13 cells were subjected to SDS PAGE followed by western blot with 22234-1-AP (ADAM28 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22234-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse small intestine using 22234-1-AP (same clone as 22234-1-PBS)

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 22234-1-AP (ADAM28 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22234-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse colon using 22234-1-AP (same clone as 22234-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 22234-1-AP (ADAM28 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22234-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse lung using 22234-1-AP (same clone as 22234-1-PBS)

  IP result of anti-ADAM28 (IP:22234-1-AP, 4ug; Detection:22234-1-AP 1:800) with mouse lung tissue lysate 3600ug. This data was developed using the same antibody clone with 22234-1-PBS in a different storage buffer formulation.

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Product Information

22234-1-PBS targets ADAM28 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADAM28 fusion protein Ag17586
• Full Name: ADAM metallopeptidase domain 28
• Calculated Molecular Weight: 775 aa, 87 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC136478
• Gene Symbol: ADAM28
• Gene ID (NCBI): 10863
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ADAM28 belongs to a family of cell surface and secreted glycoproteins that possess both proteolytic and adhesive properties. ADAM28 is predominantly expressed by carcinoma cells in the breast carcinoma tissues as protein bands of 42 kDa and 55/57 kDa, which correspond to the active forms of ADAM28s and ADAM28m, respectively.In human non-small cell lung carcinomas and breast carcinomas, ADAM28 is overexpressed predominantly by carcinoma cells, and the expression correlates with carcinoma cell proliferation and lymph node metastasis(PMID:19601836).