ADAM2 Polyclonal antibody

ADAM2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13952-1-AP

Synonyms

ADAM 2, ADAM metallopeptidase domain 2, ADAM2, Cancer/testis antigen 15, CRYN1, CRYN2, CT15, Fertilin subunit beta, FTNB, PH 30, PH 30b, PH30, PH30 beta

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Validation Data Gallery

• 

  WB analysis of mouse testis using 13952-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13952-1-AP (ADAM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 13952-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13952-1-AP (ADAM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 13952-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 13952-1-AP (ADAM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 13952-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13952-1-AP (ADAM2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse testis tissue, human testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13952-1-AP targets ADAM2 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADAM2 fusion protein Ag5063
• Full Name: ADAM metallopeptidase domain 2
• Calculated Molecular Weight: 82 kDa
• Observed Molecular Weight: 50-55 kDa, 65-75 kDa
• GenBank Accession Number: BC034957
• Gene Symbol: ADAM2
• Gene ID (NCBI): 2515
• RRID: AB_2877996
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The members of the ADAM (a disintegrin and metalloprotease) family are membrane-anchored multi-domain proteins that play prominent roles in male reproduction(PMID: 27341348). Fertilin, a heterodimeric protein complex composed of a(ADAM1) and b(ADAM2) subunits present on the sperm surface, has been believed to mediate adhesion and fusion between the sperm and egg plasma membranes. ADAM2-deficient mouse sperm is also defective in adhesion to and fusion with the egg membrane (PMID:16407235). It has 2 isoforms produced by alternative splicing and 5 glycosylation sites.