ADAM17 Polyclonal antibody

ADAM17 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 29948-1-AP

Synonyms

TACE, Snake venom-like protease, EC:3.4.24.86, Disintegrin and metalloproteinase domain-containing protein 17, cSVP

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Validation Data Gallery

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  WB analysis using 29948-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29948-1-AP (ADAM17 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HUVEC using 29948-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 29948-1-AP (ADAM17 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HUVEC cells, MCF-7 cells, SKOV-3 cells, RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below

Product Information

29948-1-AP targets ADAM17 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADAM17 fusion protein Ag32418
• Full Name: ADAM metallopeptidase domain 17
• Calculated Molecular Weight: 824 aa, 93 kDa
• Observed Molecular Weight: 90-110 kDa
• GenBank Accession Number: BC136783
• Gene Symbol: ADAM17
• Gene ID (NCBI): 6868
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The ADAMs (A Disintegrin And Metalloprotease) are multidomain transmembrane proteins. One of the first ADAMs implicated in membrane shedding is ADAM-17, which is shown to release the active form of tumor necrosis factor (TNF)-a from its precursor (PMID:18238782). ADAM17 is also named as CSVP, TACE. The full length protein has 9 glycosylation sites, a signal peptide, propeptide and 2 isoforms produced by alternative splicing.The 120-kDa form of ADAM-17 is expressed more frequently and at higher levels in primary breast carcinomas compared with normal breast tissue(PMID:17438092).

Publications

Species	Application	Title
mouse	WB	Theranostics; Neutrophil-like pH-responsive pro-efferocytic nanoparticles improve neurological recovery by promoting erythrophagocytosis after intracerebral hemorrhage; Authors - Linfeng Fan
human	WB	Nat Commun; ADAM9 promotes type I interferon-mediated innate immunity during encephalomyocarditis virus infection; Authors - Lindsey E Bazzone
human	WB	Cancer Manag Res; DSG2 and c-MYC Interact to Regulate the Expression of ADAM17 and Promote the Development of Cervical Cancer; Authors - Li-Mian Song
human,mouse	WB	Arch Biochem Biophys; Maresin1 restrains chronic inflammation and Aβ production to ameliorate Alzheimer's disease via modulating ADAM10/17 and its associated neuroprotective signal pathways: A pilot study; Authors - Shuang Sun ; KD Validated