ADAM12 Polyclonal antibody

ADAM12 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26928-1-AP

Synonyms

ADAM 12, ADAM12, MCMP, MCMPMltna, Meltrin alpha, MLTN, MLTNA

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Validation Data Gallery

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  WB analysis using 26928-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26928-1-AP (ADAM12 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 26928-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 26928-1-AP (ADAM12 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 26928-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26928-1-AP (ADAM12 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 26928-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 26928-1-AP (ADAM12 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human endometrial cancer using 26928-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 26928-1-AP (ADAM12 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 26928-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 26928-1-AP (ADAM12 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, A431 cells, A549 cells, rat brain tissue
• Positive IHC detected in: human endometrial cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26928-1-AP is ADAM12 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADAM12 fusion protein Ag17474
• Full Name: ADAM metallopeptidase domain 12
• Calculated Molecular Weight: 909 aa, 100 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC060804
• Gene Symbol: ADAM12
• Gene ID (NCBI): 8038
• RRID: AB_2880689
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADAM12, also named as MLTN and Meltrin-alpha, is involved in skeletal muscle regeneration, specifically at the onset of cell fusion. It is also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors. ADAM12 is expressed in human malignant tumors. It is involved in the regulation of growth factor activities and integrin functions, leading to promotion of cell growth and invasion, although the precise mechanisms of these are not clear at the present time.(PMID:17355265) ADAM12's ability to degrade extracellular matrix components likely allows it to detach cancer cells from the basement membrane and assist them on their route to metastasis. But the protein's role not just as a biomarker of breast cancer but as a gateway to cancer cell migration is only now being understood. ADAM12 has 2 isoforms with the calculated molecular mass of 100 and 80 kDa, and this antibody can recognize all isoforms of ADAM12. ADAM12 has 4 forms: 120kd full-length form, 90 kDa mature (processed form that lack the prodomain), 50-68 kDa degradation product and 27 kDa prodomain.