ADAM10 Monoclonal antibody

ADAM10 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A6B7

Cat no : 66620-1-Ig

Synonyms

AD10, ADAM 10, ADAM10, CD156c, CDw156, HsT18717, kuz, Kuzbanian protein homolog, MADM

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Validation Data Gallery

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  WB analysis of THP-1 using 66620-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66620-1-Ig (ADAM10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66620-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66620-1-Ig (ADAM10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 66620-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66620-1-Ig (ADAM10 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 66620-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66620-1-Ig (ADAM10 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: THP-1 cells, MCF-7 cells, Jurkat cells, K-562 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66620-1-Ig targets ADAM10 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ADAM10 fusion protein Ag23062
• Full Name: ADAM metallopeptidase domain 10
• Calculated Molecular Weight: 748 aa, 84 kDa
• Observed Molecular Weight: 90-95 kDa
• GenBank Accession Number: BC126253
• Gene Symbol: ADAM10
• Gene ID (NCBI): 102
• RRID: AB_2881980
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADAM10, a member of the ADAM (a disintegrin and metalloprotease) family, is widely expressed in the brain, the spinal cord, and the visual system during development.MW of premature ADAM10 is 90 kDa and mature ADAM10 is 60-70 kDa (PMID: 24404179).