ADAL Polyclonal antibody

ADAL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 27369-1-AP

Synonyms

ADAL, adenosine deaminase like

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Validation Data Gallery

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  WB analysis using 27369-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27369-1-AP (ADAL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of LNCaP using 27369-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 27369-1-AP (ADAL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 27369-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27369-1-AP (ADAL Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 27369-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27369-1-AP (ADAL Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human breast cancer using 27369-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27369-1-AP (ADAL Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27369-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27369-1-AP (ADAL Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, LNCaP cells, K-562 cells
• Positive IHC detected in: human heart tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

27369-1-AP targets ADAL in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADAL fusion protein Ag25949
• Full Name: adenosine deaminase-like
• Calculated Molecular Weight: 267 aa, 30 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC075857
• Gene Symbol: ADAL
• Gene ID (NCBI): 161823
• RRID: AB_2880855
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	EMBO J; Aberrant DNA N6 -methyladenine incorporation via adenylate kinase 1 is suppressed by ADAL deaminase-dependent 2'-deoxynucleotide pool sanitation; Authors - Shaokun Chen