ACVRL1 Recombinant antibody

ACVRL1 Uni-rAb^TM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241686B9

Cat no : 84421-1-RR

Synonyms

ALK1, ALK 1, ACVRLK1, Activin receptor-like kinase 1, Activin receptor like kinase 1

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Validation Data Gallery

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  IF Staining of Jurkat using 84421-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ACVRL1 antibody (84421-1-RR, Clone: 241686B9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84421-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84421-1-RR against Human ACVRL1 were performed. The affinity constant is 0.727 nM.

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Tested Applications

• Positive IF/ICC detected in: Jurkat cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84421-1-RR targets ACVRL1 in IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FusionProtein
• Full Name: activin A receptor type II-like 1
• Calculated Molecular Weight: 56 kDa
• GenBank Accession Number: BC042637
• Gene Symbol: ACVRL1
• Gene ID (NCBI): 94
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACVRL1 (also known as ALK1) is a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. ACVRL1 is highly expressed in endothelial cells and has a critical role in the control of blood vessel development and repair (PMID: 8640225). Mutations in the ACVRL1 gene are associated with hemorrhagic telangiectasia type 2.