ACVR1B Recombinant antibody

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  WB analysis using 83026-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83026-6-RR (ACVR1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 83026-6-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83026-6-RR (ACVR1B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 83026-6-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83026-6-RR (ACVR1B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of A549 using 83026-6-RR

  1x10^6 A549 cells were intracellularly stained with 0.4 ug ACVR1B Recombinant antibody (83026-6-RR, Clone:230260C1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83026-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83026-6-RR against Human ACVR1B were performed. The affinity constant is 35.6 pM.

ACVR1B Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 83026-6-RR

Clone No.230260C1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

Activin receptor-like kinase 4, Activin receptor type-1B, Activin receptor type 1B, Activin receptor like kinase 4, activin A receptor, type IB

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Tested Applications

• Positive WB detected in: Caco-2 cells, HepG2 cells, HEK-293 cells
• Positive IHC detected in: rat brain tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83026-6-RR targets ACVR1B in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ACVR1B fusion protein Ag0129
• Full Name: activin A receptor, type IB
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC000254
• Gene Symbol: ACVR1B
• Gene ID (NCBI): 91
• RRID: AB_3670766
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P36896
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACVR1B (ALK4, activin A receptor type 1B) is a transforming growth factor-beta (TGF-beta) superfamily member (PMID: 37020544). A potential role for ACVR1B in regulating muscle mass is that it is part of the transforming growth factor b (TGFb) pathway regulating myostatin (PMID: 21063444).