ARP2 Polyclonal antibody

• Featured Product
• KD/KO Validated

ARP2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 10922-1-AP

Synonyms

ACTR2, Actin-related protein 2, Actin-like protein 2

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Validation Data Gallery

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  WB analysis using 10922-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10922-1-AP (ARP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 10922-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10922-1-AP (ARP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 10922-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10922-1-AP (ARP2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 10922-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10922-1-AP (ARP2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10922-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10922-1-AP (ARP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10922-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10922-1-AP (ARP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U-251 using 10922-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using ARP2 antibody (10922-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HEK-293 using 10922-1-AP

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human ARP2 (10922-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse kidney tissue, Jurkat cells, MCF-7 cells, SH-SY5Y cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U-251 cells
• Positive FC (Intra) detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

10922-1-AP targets ARP2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARP2 fusion protein Ag1359
• Full Name: ARP2 actin-related protein 2 homolog (yeast)
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 43-45 kDa
• GenBank Accession Number: BC014546
• Gene Symbol: ARP2/ACTR2
• Gene ID (NCBI): 10097
• RRID: AB_2221854
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; miR-129-3p controls cilia assembly by regulating CP110 and actin dynamics.; Authors - Cao Jingli J ; KD Validated
human	WB	Nucleic Acids Res; G3BP1 binds to guanine quadruplexes in mRNAs to modulate their stabilities.; Authors - Xiaomei He
	WB	Elife; Syncytin-mediated open-ended membrane tubular connections facilitate the intercellular transfer of cargos including Cas9 protein; Authors - Congyan Zhang
human	WB	Elife; Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors.; Authors - Dawei Wang ; KD Validated
human	IF	Elife; mTOR signaling regulates the morphology and migration of outer radial glia in developing human cortex.; Authors - Madeline G Andrews
mouse	WB	Biol Reprod; RAB8A GTPase regulates spindle migration and Golgi apparatus distribution via ROCK-mediated actin assembly in mouse oocyte meiosis.; Authors - Zhen-Nan Pan