ACTN3 Recombinant antibody, PBS Only

ACTN3 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241168A5

Cat no : 84356-1-PBS

Synonyms

Alpha-actinin-3, Alpha-actinin skeletal muscle isoform 3, Alpha actinin 3, ACTN 3, actinin, alpha 3

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Validation Data Gallery

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  WB analysis of rat skeletal muscle using 84356-1-RR (same clone as 84356-1-PBS)

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 84356-1-RR (ACTN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84356-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 84356-1-RR (same clone as 84356-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84356-1-RR (ACTN3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84356-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse skeletal muscle using 84356-1-RR (same clone as 84356-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using ACTN3 antibody (84356-1-RR, Clone: 241168A5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84356-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84356-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84356-1-RR against Human ACTN3 were performed. The affinity constant is 0.116 nM.

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Product Information

The immunogen of 84356-1-PBS is ACTN3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ACTN3 fusion protein Ag36709
• Full Name: actinin, alpha 3
• Calculated Molecular Weight: 901 aa, 103 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC099647
• Gene Symbol: ACTN3
• Gene ID (NCBI): 89
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.