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ACTN2 Monoclonal antibody, PBS Only

ACTN2 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D5F9

Cat no : 68223-1-PBS

Synonyms

actinin, alpha 2, ACTN2, Alpha actinin 2, CMD1AA, F actin cross linking protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACTN2 Monoclonal antibody (68223-1-Ig)

    WB analysis using 68223-1-Ig (same clone as 68223-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68223-1-Ig (ACTN2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68223-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat colon tissue using ACTN2 Monoclonal antibody (68223-1-Ig)

    WB analysis of rat colon using 68223-1-Ig (same clone as 68223-1-PBS)

    rat colon tissue were subjected to SDS PAGE followed by western blot with 68223-1-Ig (ACTN2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68223-1-PBS in a different storage buffer formulation.

Product Information

68223-1-PBS targets ACTN2 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Reactivity Human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ACTN2 fusion protein Ag5578
Full Name actinin, alpha 2
Calculated Molecular Weight 104 kDa
Observed Molecular Weight 103 kDa
GenBank Accession NumberBC051770
Gene Symbol ACTN2
Gene ID (NCBI) 88
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Alpha actinin 2 (ACTN2) belongs to the alpha-actinin family and is expressed in both skeletal and cardiac muscles and functions to anchor myofibrillar actin thin filaments and titin to Z-discs (PMID: 30701273). ACTN2 is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. Mutations in ACTN2 are associated with hypertrophic cardiomyopathy, as well as dilated cardiomyopathy and endocardial fibroelastosis (PMID: 20022194, 14567970).