ACTG2 Polyclonal antibody

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  WB analysis using 31849-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31849-1-AP (ACTG2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 31849-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31849-1-AP (ACTG2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

ACTG2 Polyclonal Antibody for WB, ELISA

Cat No. 31849-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ACTA3, Actin, gamma-enteric smooth muscle, Actin, gamma-enteric smooth muscle, intermediate form, ACTL3, ACTSG

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Tested Applications

• Positive WB detected in: HeLa cells, mouse skeletal muscle tissue, HepG2 cells, mouse stomach tissue, rat skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31849-1-AP targets ACTG2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACTG2 fusion protein Ag36024
• Full Name: actin, gamma 2, smooth muscle, enteric
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC094877
• Gene Symbol: ACTG2
• Gene ID (NCBI): 72
• RRID: AB_3670128
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P63267
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Actin gamma 2 (ACTG2, also known as ACT, ACTE, VSCM, ACTA3, ACTL3, and ACTSG) is a highly conserved protein involved in various types of cell motility and ubiquitously expressed in all eukaryotic cells. ACTG2 mutations would cause congenital distended bladder, microcolon, and intestinal hypoperistalsis (PMID: 24337657). It also has been reported that ACTG2 promotes hepatocellular carcinoma (HCC) migration and invasion through the Notch1 signaling pathway, which might serve as a new intervention target for the treatment of HCC (PMID: 28385530).