Alpha Actin Polyclonal antibody

Alpha Actin Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 23082-1-AP

Synonyms

Alpha 1 actin, Alpha actin

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Validation Data Gallery

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  WB analysis of mouse heart using 23082-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 23082-1-AP (alpha Actin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 23082-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 23082-1-AP (alpha Actin antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 23082-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 23082-1-AP (alpha Actin antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 23082-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 23082-1-AP (alpha Actin antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human small intestine using 23082-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 23082-1-AP (alpha Actin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human small intestine using 23082-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 23082-1-AP (alpha Actin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 23082-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 23082-1-AP (alpha Actin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 23082-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 23082-1-AP (alpha Actin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse skeletal muscle using 23082-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 23082-1-AP (alpha Actin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 23082-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 23082-1-AP (alpha Actin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 23082-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 23082-1-AP (alpha Actin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 23082-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 23082-1-AP (alpha Actin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 23082-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 23082-1-AP (alpha Actin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 23082-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 23082-1-AP (alpha Actin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse colon using 23082-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 23082-1-AP (alpha Actin antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse colon using 23082-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 23082-1-AP (alpha Actin antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon using 23082-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 23082-1-AP (alpha Actin antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon using 23082-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 23082-1-AP (alpha Actin antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 23082-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 23082-1-AP (alpha Actin antibody) at dilution of 1:100 (under 40x lens).

• 

  FC experiment of C2C12 using 23082-1-AP

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human Alpha Actin (23082-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: mouse heart tissue
• Positive IHC detected in: human heart tissue, mouse skeletal muscle tissue, mouse heart tissue, mouse colon tissue, human small intestine tissue, human colon tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: C2C12 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 23082-1-AP is Alpha Actin Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Alpha Actin fusion protein Ag19482
• Full Name: actin, alpha, cardiac muscle 1
• Calculated molecular weight: 377 aa, 42 kDa
• Observed molecular weight: 42-45 kDa
• GenBank accession number: BC009978
• Gene symbol: ACTC1
• Gene ID (NCBI): 70
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
	WB	Circ Heart Fail; Phenotype and Physiological Significance of the Endocardial Smooth Muscle Cells in Human Failing Hearts.; Authors - Hideshi Okada

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C