Tested Applications
| Positive WB detected in | mouse colon tissue, mouse kidney tissue, mouse stomach, rat colon, mouse lung, rat lung |
| Positive IHC detected in | mouse heart tissue, human colon tissue, human colon cancer tissue, human heart tissue, human hepatocirrhosis tissue, human liver cancer tissue, mouse colon tissue, mouse liver tissue, rat colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:3000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 193 publications below |
| IHC | See 75 publications below |
| IF | See 124 publications below |
Product Information
55135-1-AP targets Alpha smooth muscle actin specific in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, rabbit |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | actin, alpha 2, smooth muscle, aorta |
| Calculated Molecular Weight | 42 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | NM_001613 |
| Gene Symbol | Alpha smooth muscle actin |
| Gene ID (NCBI) | 59 |
| RRID | AB_10949628 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P62736 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ACTA2, also named as a-SMA,AAT6;ACTSA belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6). The immunogen of this antibody is a specific domain of ACTA2. This antibody stains the aorta only.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Alpha smooth muscle actin specific antibody 55135-1-AP | Download protocol |
| IHC protocol for Alpha smooth muscle actin specific antibody 55135-1-AP | Download protocol |
| IF protocol for Alpha smooth muscle actin specific antibody 55135-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
ACS Nano Cysteine-Based Redox-Responsive Nanoparticles for Fibroblast-Targeted Drug Delivery in the Treatment of Myocardial Infarction | ||
Cancer Commun (Lond) Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer | ||
Adv Sci (Weinh) LIMA1 O-GlcNAcylation Promotes Hepatic Lipid Deposition through Inducing β-catenin-Regulated FASn Expression in Metabolic Dysfunction-Associated Steatotic Liver Disease | ||
Nat Commun Copper sulfide nanoparticles as a photothermal switch for TRPV1 signaling to attenuate atherosclerosis. | ||
Mol Cancer Extracellular vesicles of carcinoma-associated fibroblasts creates a pre-metastatic niche in the lung through activating fibroblasts. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH S (Verified Customer) (09-23-2024) | Excelllent
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FH PK (Verified Customer) (03-22-2022) |
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FH S (Verified Customer) (12-01-2021) | Excellent
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