smooth muscle actin specific Polyclonal antibody
smooth muscle actin specific Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat and More (1)
WB, IHC, IF, ELISA
Cat no : 55135-1-AP
Validation Data Gallery
|Positive WB detected in||mouse colon tissue, mouse kidney tissue, mouse stomach, rat colon, mouse lung, rat lung|
|Positive IHC detected in||human heart tissue, human liver cancer tissue, human hepatocirrhosis tissue, human colon tissue, human colon cancer tissue, rat colon tissue, mouse colon tissue, mouse liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse heart tissue|
|Western Blot (WB)||WB : 1:500-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|WB||See 137 publications below|
|IHC||See 48 publications below|
|IF||See 83 publications below|
55135-1-AP targets smooth muscle actin specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, rabbit|
|Host / Isotype||Rabbit / IgG|
|Full Name||actin, alpha 2, smooth muscle, aorta|
|Calculated molecular weight||42 kDa|
|Observed molecular weight||42 kDa|
|GenBank accession number||NM_001613|
|Gene ID (NCBI)||59|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
ACTA2, also named as a-SMA,AAT6;ACTSA belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6). The immunogen of this antibody is a specific domain of ACTA2. This antibody stains the aorta only.
|Product Specific Protocols|
|WB protocol for smooth muscle actin specific antibody 55135-1-AP||Download protocol|
|IHC protocol for smooth muscle actin specific antibody 55135-1-AP||Download protocol|
|IF protocol for smooth muscle actin specific antibody 55135-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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PK (Verified Customer) (03-22-2022)
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