Alpha smooth muscle actin specific Polyclonal antibody

Alpha smooth muscle actin specific Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

266

Reviews (3)

Cat no : 55135-1-AP

Synonyms

ACTA2, Alpha SMA, a-SMA, smooth muscle actin, Alpha actin 2

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Validation Data Gallery

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  WB analysis using 55135-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55135-1-AP (smooth muscle actin-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 55135-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55135-1-AP (smooth muscle actin specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 55135-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 55135-1-AP (Alpha SMA-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55135-1-AP (ACTA2-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55135-1-AP (ACTA2-Specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver cancer using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55135-1-AP (smooth muscle actin specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55135-1-AP (smooth muscle actin specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 55135-1-AP (smooth muscle actin specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 55135-1-AP (ACTA2-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 55135-1-AP (ACTA2-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55135-1-AP (ACTA2-Specific antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon cancer using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55135-1-AP (Alpha SMA-specific antibody at dilution of 1:2000 (under 10x lens).

• 

  IHC staining of human colon cancer using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55135-1-AP (Alpha SMA-specific antibody at dilution of 1:2000 (under 40x lens).

• 

  IHC staining of human colon using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55135-1-AP (Alpha SMA-specific antibody at dilution of 1:2000 (under 10x lens).

• 

  IHC staining of human colon using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55135-1-AP (Alpha SMA-specific antibody at dilution of 1:2000 (under 40x lens).

• 

  IHC staining of mouse colon using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 55135-1-AP (Alpha SMA-specific antibody) at dilution of 1:2000 (under 10x lens).

• 

  IHC staining of mouse colon using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 55135-1-AP (Alpha SMA-specific antibody) at dilution of 1:2000 (under 40x lens).

• 

  IHC staining of human hepatocirrhosis using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 55135-1-AP (ACTA2-Specific antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 55135-1-AP (ACTA2-Specific antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse liver using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 55135-1-AP (Alpha SMA-specific antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 55135-1-AP (Alpha SMA-specific antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 55135-1-AP (Alpha SMA-specific antibody at dilution of 1:100 (under 10x lens).

• 

  IHC staining of rat colon using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 55135-1-AP (Alpha SMA-specific antibody at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55135-1-AP (ACTA2-Specific antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of mouse heart using 55135-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 55135-1-AP (Alpha SMA-specific antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 55135-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 55135-1-AP (Alpha SMA-specific antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse colon tissue, mouse kidney tissue, mouse stomach, rat colon, mouse lung, rat lung
• Positive IHC detected in: mouse heart tissue, human colon tissue, human colon cancer tissue, human heart tissue, human hepatocirrhosis tissue, human liver cancer tissue, mouse colon tissue, mouse liver tissue, rat colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:4000
• Immunohistochemistry (IHC): IHC : 1:500-1:3000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 177 publications below
• IHC: See 67 publications below
• IF: See 116 publications below

Product Information

55135-1-AP targets Alpha smooth muscle actin specific in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, rabbit
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: actin, alpha 2, smooth muscle, aorta
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: NM_001613
• Gene Symbol: Alpha smooth muscle actin
• Gene ID (NCBI): 59
• RRID: AB_10949628
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACTA2, also named as a-SMA,AAT6;ACTSA belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6). The immunogen of this antibody is a specific domain of ACTA2. This antibody stains the aorta only.

Publications

Species	Application	Title
mouse	IF	Nat Cell Biol; Ex vivo imaging reveals the spatiotemporal control of ovulation; Authors - Christopher Thomas
mouse	IF	ACS Nano; Cysteine-Based Redox-Responsive Nanoparticles for Fibroblast-Targeted Drug Delivery in the Treatment of Myocardial Infarction; Authors - Xiaoqian Ji
human	IHC	Cancer Commun (Lond); Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer; Authors - Xingbin Hu
mouse	IF	Nat Commun; Copper sulfide nanoparticles as a photothermal switch for TRPV1 signaling to attenuate atherosclerosis.; Authors - Wen Gao
human	IF	Mol Cancer; Extracellular vesicles of carcinoma-associated fibroblasts creates a pre-metastatic niche in the lung through activating fibroblasts.; Authors - Jing Kong
mouse	WB,IF	J Nanobiotechnology; Evolocumab loaded Bio-Liposomes for efficient atherosclerosis therapy; Authors - Zhenxian Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

S (Verified Customer) (09-23-2024)

Excelllent

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: A549

Alpha smooth muscle actin specific Antibody Western Blot validation (1000 dilution) in A549 (Cat no:55135-1-AP)

FH

PK (Verified Customer) (03-22-2022)

• Applications: Western Blot
• Cell Tissue Type: cardiac Fibroblast

Alpha smooth muscle actin specific Antibody Western Blot validation ( dilution) in cardiac Fibroblast (Cat no:55135-1-AP)

FH

S (Verified Customer) (12-01-2021)

Excellent

• Applications: Western Blot
• Cell Tissue Type: LUNG FIBROBLASTS

Alpha smooth muscle actin specific Antibody Western Blot validation ( dilution) in LUNG FIBROBLASTS (Cat no:55135-1-AP)

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