Validation Data Gallery
|Positive WB detected in||mouse heart tissue, mouse brown adipose tissue, mouse kidney tissue, mouse liver tissue, mouse skeletal muscle tissue, rat heart tissue|
|Positive IP detected in||mouse heart tissue|
|Positive IHC detected in||human heart tissue, mouse skeletal muscle tissue, human colon tissue, human liver cancer tissue, stromal tumor tissue, human hysteromyoma tissue, human liver tissue, human small intestine tissue, human skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:200-1:1000|
|Sample-dependent, check data in validation data gallery|
The immunogen of 23081-1-AP is smooth muscle actin Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||smooth muscle actin fusion protein Ag19481|
|Full Name||actin, alpha 2, smooth muscle, aorta|
|Calculated molecular weight||377 aa, 42 kDa|
|Observed molecular weight||42 kDa|
|GenBank accession number||BC017554|
|Gene ID (NCBI)||59|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. This antibody is specific to the ACTA2. It selectively stains the vascular smooth muscle but not cardiac or skeletal muscle.
|Product Specific Protocols|
|WB protocol for smooth muscle actin antibody 23081-1-AP||Download protocol|
|IHC protocol for smooth muscle actin antibody 23081-1-AP||Download protocol|
|IP protocol for smooth muscle actin antibody 23081-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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