smooth muscle actin Polyclonal antibody

smooth muscle actin Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

35

Cat no : 23081-1-AP

Synonyms

a SMA, AAT6, ACTA2, ACTA2 Smooth muscle Actin, ACTA2 specific, Actin, aortic smooth muscle, ACTSA, ACTVS, Alpha actin 2, Alpha SMA, a-SMA, smooth muscle actin, α SMA, α-sma

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Validation Data Gallery

• 

  WB analysis of mouse heart using 23081-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brown adipose using 23081-1-AP

  mouse brown adipose tissue were subjected to SDS PAGE followed by western blot with 23081-1-AP (a-SMA specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 23081-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 23081-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 23081-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 23081-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 23081-1-AP

  IP result of anti-smooth muscle actin (IP:23081-1-AP, 4ug; Detection:23081-1-AP 1:1000) with mouse heart tissue lysate 3200ug.

• 

  IHC staining of human heart using 23081-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:500 (under 10x lens).

• 

  IHC staining of human colon using 23081-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:6400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 23081-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:500 (under 40x lens).

• 

  IHC staining of human liver cancer using 23081-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:3200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 23081-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:3200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of stromal tumor using 23081-1-AP

  Immunohistochemical analysis of paraffin-embedded stromal tumor tissue slide using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:3200 (under 10x lens).

• 

  IHC staining of stromal tumor using 23081-1-AP

  Immunohistochemical analysis of paraffin-embedded stromal tumor tissue slide using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:3200 (under 40x lens).

• 

  IHC staining of human hysteromyoma using 23081-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:3200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hysteromyoma using 23081-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:3200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 23081-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 23081-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 23081-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 23081-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 23081-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:500 (under 10x lens).

• 

  IHC staining of human small intestine using 23081-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:500 (under 40x lens).

• 

  IHC staining of human colon using 23081-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:6400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 23081-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:500 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 23081-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:500 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse brown adipose tissue, mouse kidney tissue, mouse liver tissue, mouse skeletal muscle tissue, rat heart tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human heart tissue, mouse skeletal muscle tissue, human colon tissue, human liver cancer tissue, stromal tumor tissue, human hysteromyoma tissue, human liver tissue, human small intestine tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 21 publications below
• IHC: See 11 publications below
• IF: See 6 publications below

Product Information

The immunogen of 23081-1-AP is smooth muscle actin Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: smooth muscle actin fusion protein Ag19481
• Full Name: actin, alpha 2, smooth muscle, aorta
• Calculated Molecular Weight: 377 aa, 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC017554
• Gene Symbol: Alpha smooth muscle actin
• Gene ID (NCBI): 59
• RRID: AB_2815024
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. This antibody is specific to the ACTA2. It selectively stains the vascular smooth muscle but not cardiac or skeletal muscle.

Publications

Species	Application	Title
mouse	IF	Nat Commun; Dental cell type atlas reveals stem and differentiated cell types in mouse and human teeth.; Authors - Jan Krivanek
human	WB	Drug Des Devel Ther; Keloid Patient Plasma-Derived Exosomal hsa_circ_0020792 Promotes Normal Skin Fibroblasts Proliferation, Migration, and Fibrogenesis via Modulating miR-193a-5p and Activating TGF-β1/Smad2/3 Signaling; Authors - Huan Hu
mouse	WB	Biofabrication; Antibacterial, ROS scavenging and angiogenesis promotingϵ-Polylysine/gelatin based hydrogel containing CTLP to regulate macrophages for pressure ulcer healing; Authors - PinLi Lin
mouse	IHC	Cardiovasc Res; Plasma levels of trimethylamine-N-oxide can be increased with 'healthy' and 'unhealthy' diets and do not correlate with the extent of atherosclerosis but with plaque instability.; Authors - Yen Chin Koay
rat	WB	Environ Pollut; Polystyrene microplastics cause cardiac fibrosis by activating Wnt/β-catenin signaling pathway and promoting cardiomyocyte apoptosis in rats.; Authors - Zekang Li
rat	IHC	Cardiovasc Res; PMID: 23650288; Authors - Fan You-Fei YF

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