ACSS3 Recombinant antibody

ACSS3 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242423C6

Cat no : 84810-4-RR

Synonyms

EC:6.2.1.1, Acyl-CoA synthetase short-chain family member 3, mitochondrial, Acyl-CoA synthetase short-chain family member 3, ACSS 3, Acetate--CoA ligase 3

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Validation Data Gallery

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  WB analysis of HepG2 using 84810-4-RR

  HepG2 cells were subjected to SDS PAGE followed by western blot with 84810-4-RR (ACSS3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of THP-1 using 84810-4-RR

  THP-1 cells were subjected to SDS PAGE followed by western blot with 84810-4-RR (ACSS3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HepG2 cells, THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84810-4-RR targets ACSS3 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ACSS3 fusion protein Ag9173
• Full Name: acyl-CoA synthetase short-chain family member 3
• Calculated Molecular Weight: 686 aa, 75 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC009317
• Gene Symbol: ACSS3
• Gene ID (NCBI): 79611
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACSS3(acyl-CoA synthetase short-chain family member 3, mitochondrial) belongs to the ATP-dependent AMP-binding enzyme family. It activates acetate so that it can be used for lipid synthesis or for energy generation. The deduced 686-amino acid protein contains 4 of 5 motifs characteristic of acyl-CoA synthetases. This protein has 2 isoforms produced by alternative splicing. The full length protein has a transit peptide with 29 amino acids.