ACSS2 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

ACSS2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16087-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 16087-1-AP (same clone as 16087-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16087-1-AP (ACSS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16087-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 16087-1-AP (same clone as 16087-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16087-1-AP (ACSS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16087-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 16087-1-AP (same clone as 16087-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16087-1-AP (ACSS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16087-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 16087-1-AP (same clone as 16087-1-PBS)

  IP result of anti-ACSS2 (IP:16087-1-AP, 4ug; Detection:16087-1-AP 1:500) with HepG2 cells lysate 5600 ug. This data was developed using the same antibody clone with 16087-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 16087-1-AP (same clone as 16087-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16087-1-AP (ACSS2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16087-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 16087-1-AP (same clone as 16087-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16087-1-AP (ACSS2 synthetase antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16087-1-PBS in a different storage buffer formulation.

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Product Information

16087-1-PBS targets ACSS2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACSS2 fusion protein Ag9121
• Full Name: acyl-CoA synthetase short-chain family member 2
• Calculated Molecular Weight: 701 aa, 79 kDa
• Observed Molecular Weight: 79 kDa
• GenBank Accession Number: BC010141
• Gene Symbol: ACSS2
• Gene ID (NCBI): 55902
• RRID: AB_2878216
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Acetyl-CoA Synthetase 2 (ACSS2) is also named as acyl-CoA synthetase short-chain family member 2. Acetyl-CoA Synthetase 2 (ACSS2) is responsible for lipogenesis by synthesizing acetyl-CoA from acetate for lipogenesis and promotes acetylation in nuclear (PMID: 29444517). It is also required for cell migration and invasion in renal cell carcinoma (RCC) through the promotion of autophagy (PMID: 29281714). ACSS2 has two isoforms with the molecular weight of 78 and 80 kDa.