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ACSS1 Monoclonal antibody, PBS Only

ACSS1 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E10F11

Cat no : 68608-1-PBS

Synonyms

ACAS2L, AceCS2, AceCS2L, Acetate CoA ligase 2, Acetyl CoA synthetase 2, ACSS1, FLJ45659, KIAA1846

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACSS1 Monoclonal antibody (68608-1-Ig)

    WB analysis using 68608-1-Ig (same clone as 68608-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68608-1-Ig (ACSS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68608-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ACSS1 Monoclonal antibody (68608-1-Ig)

    WB analysis using 68608-1-Ig (same clone as 68608-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68608-1-Ig (ACSS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68608-1-PBS in a different storage buffer formulation.

Product Information

68608-1-PBS targets ACSS1 in WB, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ACSS1 fusion protein Ag10853
Full Name acyl-CoA synthetase short-chain family member 1
Calculated Molecular Weight 689 aa, 75 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC039261
Gene Symbol ACSS1
Gene ID (NCBI) 84532
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The ACSS (acetyl-CoA synthetase) enzyme is the sole known mammalian enzyme that can catalyze the conversion of free acetate into acetyl coenzyme A (acetyl-CoA). The three known isoforms of human ACSS are termed ACSS1, ACSS2, and ACSS3. The main substrate of ACSS1 and ACSS2 is acetate, while the preferential substrate of ACSS3 is propionate. Two acetate related enzymes, ACSS1(GeneID: 84532) and ACSS2 (GeneID:55902) difer in their tissue distribution and subcellular localization. On the one hand, as a mitochondrial matrix enzyme, ACSS1 is expressed mainly in cardiac and skeletal muscle as well as brown adipose tissue. On the other hand, as a nuclear and cytoplasmic enzyme, ACSS2 is strongly expressed in the liver, kidney and heart and moderately expressed in the brain and testis.ACSS2 participates in lipid synthesis and facilitates protein acetylation by generating acetyl-CoA, while ACSS1 is involved in acetate oxidation. The functional diferences in these enzymes involve energy production through the tricarboxylic acid (TCA) cycle. Due to its more thorough utilization of intracellular acetate, ACSS2 is expressed in almost all cell types under diferent physiological conditions.