ACSM5 Monoclonal antibody, PBS Only

ACSM5 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D5D6

Cat no : 67334-1-PBS

Synonyms

ACSM5, FLJ20581, MACS3

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Validation Data Gallery

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  WB analysis of 4T1 using 67334-1-Ig (same clone as 67334-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67334-1-Ig (ACSM5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 67334-1-Ig (same clone as 67334-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67334-1-Ig (ACSM5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.

• 

  WB analysis of 4T1 using 67334-1-Ig (same clone as 67334-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67334-1-Ig (ACSM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MDA-MB-231 using 67334-1-Ig (same clone as 67334-1-PBS)

  MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 67334-1-Ig (ACSM5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67334-1-Ig (same clone as 67334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67334-1-Ig (ACSM5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67334-1-Ig (same clone as 67334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67334-1-Ig (ACSM5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67334-1-Ig (same clone as 67334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67334-1-Ig (ACSM5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67334-1-Ig (same clone as 67334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67334-1-Ig (ACSM5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67334-1-Ig (same clone as 67334-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ACSM5 antibody (67334-1-Ig, Clone: 2D5D6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.

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Product Information

67334-1-PBS targets ACSM5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ACSM5 fusion protein Ag10056
• Full Name: acyl-CoA synthetase medium-chain family member 5
• Calculated Molecular Weight: 208 aa, 23 kDa
• Observed Molecular Weight: 60-65 kDa
• GenBank Accession Number: BC016703
• Gene Symbol: ACSM5
• Gene ID (NCBI): 54988
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ACSM5(Acyl-CoA synthetase medium-chain family member 5) is also named as MACS3 and belongs to the ATP-dependent AMP-binding enzyme family. ACSM5 has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). This protein has 2 isoforms produced by alternative splicing.