ACSM3 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

ACSM3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10168-2-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HEK-293 using 10168-2-AP (same clone as 10168-2-PBS)

  WB result of ACSM3 antibody (10168-2-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACSM3 transfected HEK-293 cells. This data was developed using the same antibody clone with 10168-2-PBS in a different storage buffer formulation.

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  WB analysis using 10168-2-AP (same clone as 10168-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10168-2-AP (ACSM3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10168-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 10168-2-AP (same clone as 10168-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10168-2-AP (ACSM3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10168-2-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 10168-2-AP (same clone as 10168-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10168-2-AP (ACSM3 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10168-2-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 10168-2-AP (same clone as 10168-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10168-2-AP (ACSM3 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10168-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 10168-2-AP (same clone as 10168-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ACSM3 antibody (10168-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 10168-2-PBS in a different storage buffer formulation.

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Product Information

10168-2-PBS targets ACSM3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACSM3 fusion protein Ag0224
• Full Name: acyl-CoA synthetase medium-chain family member 3
• Calculated Molecular Weight: 49 kDa, 66 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC002790
• Gene Symbol: ACSM3
• Gene ID (NCBI): 6296
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SA hypertension-associated rat homolog (SAH, synonym: SA) is expressed at 10-fold greater levels in the kidney of the spontaneously hypertensive rat than in the corresponding wild-type strain. The gene is linked to blood pressure levels in a number of crosses involving the spontaneously hypertensive rat and other strains of genetically hypertensive rats. Human SAH gene polymorphism may be associated with the renal prognosis of immunoglobulin A nephropathy through its effect on blood pressure. Furthermore, variation in SAH has a role in predisposition.