ACSM2A Polyclonal antibody, PBS Only

ACSM2A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 22862-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 22862-1-AP (same clone as 22862-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22862-1-AP (ACSM2A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22862-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 22862-1-AP (same clone as 22862-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 22862-1-AP (ACSM2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22862-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 22862-1-AP (same clone as 22862-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22862-1-AP (ACSM2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22862-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 22862-1-AP (same clone as 22862-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22862-1-AP (ACSM2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22862-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 22862-1-AP (same clone as 22862-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 22862-1-AP (ACSM2A antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22862-1-PBS in a different storage buffer formulation.

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Product Information

22862-1-PBS targets ACSM2A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACSM2A fusion protein Ag18856
• Full Name: acyl-CoA synthetase medium-chain family member 2A
• Calculated Molecular Weight: 577 aa, 64 kDa
• Observed Molecular Weight: 60-64 kDa
• GenBank Accession Number: BC125176
• Gene Symbol: ACSM2A
• Gene ID (NCBI): 123876
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ACSM2A(Acyl-coenzyme A synthetase ACSM2A, mitochondrial) is also named as ACSM2, MACS2 and belongs to the ATP-dependent AMP-binding enzyme family. It acts as a medium-chain fatty acid : CoA ligase with the caculated molecular weight of 64 kDa and acts on acids from C(4) to C(11) and on the corresponding 3-hydroxy- and 2,3- or 3,4-unsaturated acids.