ACPP Polyclonal antibody

ACPP Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 15840-1-AP

Synonyms

acid phosphatase, prostate, ACP 3, ACP3, ACPP, PAP, Prostatic acid phosphatase

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Validation Data Gallery

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  WB analysis of PC-3 using 15840-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15840-1-AP (ACPP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 15840-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15840-1-AP (ACPP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 15840-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 15840-1-AP (ACPP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 15840-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15840-1-AP (ACPP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 15840-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15840-1-AP (ACPP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 15840-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15840-1-AP (ACPP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 15840-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15840-1-AP (ACPP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate hyperplasia using 15840-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 15840-1-AP (ACPP Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate hyperplasia using 15840-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 15840-1-AP (ACPP Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 15840-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15840-1-AP (ACPP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 15840-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15840-1-AP (ACPP antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of PC-3 using 15840-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 15840-1-AP (ACPP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: PC-3 cells, COLO 320 cells, MCF-7 cells, mouse lung tissue, mouse testis tissue
• Positive IHC detected in: human prostate cancer tissue, human prostate hyperplasia tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

15840-1-AP targets ACPP in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse, rat, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACPP fusion protein Ag8592
• Full Name: acid phosphatase, prostate
• Calculated Molecular Weight: 418 aa, 48 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC007460
• Gene Symbol: ACPP
• Gene ID (NCBI): 55
• RRID: AB_2222099
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACPP is also named as PAP(prostatic acid phosphatase),5'-nucleotidase, thiamine monophosphatas and Belongs to the histidine acid phosphatase family.This enzyme is a homodimeric glycoprotein with a subunit molecular mass of 50 kDa and is an androgen-stimulated secretory product of the human prostate gland(PMID:8132635).It is also a non-specific phosphatase and has been implicated in the progression of prostate cancer, which inactivates LPA in human seminal plasma(PMID:15280042). This protein has three glycosylation sites(PMID:10639192;12525165).

Publications

Species	Application	Title
chicken	IHC	Biol Reprod; Avian Prostatic Acid Phosphatase: Estrogen Regulation in the Oviduct and Epithelial Cell-Derived Ovarian Carcinomas.; Authors - Hyocheol Bae
mouse	IHC	Am J Pathol; PIK3CG Is a Potential Therapeutic Target in Androgen Receptor-Indifferent Metastatic Prostate Cancer.; Authors - Wen-Cheng Chung
mouse	WB,IF,IP	Eur J Pain; Electroacupuncture relieves neuropathic pain by inhibiting degradation of the ecto-nucleotidase PAP in the dorsal root ganglions of CCI mice.; Authors - Xiaoxin Zhou
Rat	IHC, IF	Biochem Biophys Res Commun; Dietary cholesterol affects expression of prostatic acid phosphatase in reproductive organs of male rats.; Authors - Whasun Lim