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ACOX3 Recombinant antibody, PBS Only

ACOX3 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241172G8

Cat no : 84027-4-PBS

Synonyms

EC:1.3.3.6, BRCOX, BRCACox, Branched-chain acyl-CoA oxidase, ACOX 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACOX3 Recombinant antibody (84027-4-RR)

    WB analysis using 84027-4-RR (same clone as 84027-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84027-4-RR (ACOX3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84027-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using ACOX3 Recombinant antibody (84027-4-RR)

    FC experiment of A431 using 84027-4-RR (same clone as 84027-4-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug ACOX3 Recombinant antibody (84027-4-RR, Clone:241172G8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84027-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84027-4-RR

    Affinity and Kinetic Characterization of 84027-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84027-4-RR against Human ACOX3 were performed. The affinity constant is 1.05 nM.

Product Information

The immunogen of 84027-4-PBS is ACOX3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ACOX3 fusion protein Ag10553
Full Name acyl-Coenzyme A oxidase 3, pristanoyl
Calculated Molecular Weight 624 aa, 70 kDa
Observed Molecular Weight70-78 kDa
GenBank Accession NumberBC017053
Gene Symbol ACOX3
Gene ID (NCBI) 8310
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Acyl-Coenzyme A oxidase 3 also know as pristanoyl -CoA oxidase (ACOX3) is involved in the desaturation of 2-methyl branched fatty acids in peroxisomes.