ACOT7 Polyclonal antibody

• Featured Product
• KD/KO Validated

ACOT7 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 15972-1-AP

Synonyms

ACH1, ACOT7, ACT, acyl CoA thioesterase 7, BACH, Brain acyl CoA hydrolase, CTE II, CTE IIa, hBACH, LACH, LACH1

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Validation Data Gallery

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  WB analysis using 15972-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15972-1-AP (ACOT7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15972-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15972-1-AP (ACOT7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15972-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15972-1-AP (ACOT7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15972-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15972-1-AP (ACOT7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293T cells, mouse brain tissue, human brain tissue, Jurkat cells, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:3000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below

Product Information

15972-1-AP targets ACOT7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACOT7 fusion protein Ag8759
• Full Name: acyl-CoA thioesterase 7
• Calculated Molecular Weight: 370 aa, 40 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC017365
• Gene Symbol: ACOT7
• Gene ID (NCBI): 11332
• RRID: AB_2221652
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH (PubMed:10578051).  Acyl-coenzyme A thioesterase 7/ACOT7 preferentially hydrolyzes palmitoyl-CoA, but has a broad specificity acting on other fatty acyl-CoAs with chain-lengths of C8-C18 (PubMed:10578051). May play an important physiological function in brain (PubMed:10578051). ACOT7 has 7 isoforms between 27-40 kDa, we can detect a band at 37 kDa. ACOT7 is high homology with ACOT7L, this antibody may also detect ACOT7L.

Publications

Species	Application	Title
		Nat Commun; Convergent genomic signatures of flight loss in birds suggest a switch of main fuel.; Authors - Shengkai Pan
human	WB,IHC	J Hepatocell Carcinoma; Acyl-CoA Thioesterase 7 is Transcriptionally Activated by Krüppel-Like Factor 13 and Promotes the Progression of Hepatocellular Carcinoma.; Authors - Xingming Xie
mouse	WB	J Proteomics; TMT-labeled quantitative malonylome analysis on the longissimus dorsi muscle of Laiwu pigs reveals the role of ACOT7 in fat deposition; Authors - Wenlei Wang
human	WB,IHC	Acta Histochem; ACOT7 positively regulated by CREB1 promotes the progression of cutaneous melanoma; Authors - Ni Tang
mouse	WB	In Vitro Cell Dev Biol Anim; ACOT7 protects epidermal stem cells against lipid peroxidation; Authors - Guang Zhang
human	WB	BMC Mol Cell Biol; ARNTL2 upregulation of ACOT7 promotes NSCLC cell proliferation through inhibition of apoptosis and ferroptosis; Authors - Tao Wang ; KD Validated