ACOT6 Polyclonal antibody

ACOT6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22936-1-AP

Synonyms

ACOT6, acyl CoA thioesterase 6, c14_5530, C14orf42

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Validation Data Gallery

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  WB analysis of mouse liver using 22936-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 22936-1-AP (ACOT6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 22936-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22936-1-AP (ACOT6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 22936-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22936-1-AP (ACOT6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 22936-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 22936-1-AP (ACOT6 Antibody) at dilution of 1:50.

• 

  IHC staining of human kidney using 22936-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 22936-1-AP (ACOT6 Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: mouse liver tissue, HeLa cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22936-1-AP is ACOT6 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACOT6 fusion protein Ag18981
• Full Name: acyl-CoA thioesterase 6
• Calculated Molecular Weight: 207 aa, 23 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC126380
• Gene Symbol: ACOT6
• Gene ID (NCBI): 641372
• RRID: AB_11182380
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.