ACOT4 Polyclonal antibody, PBS Only

ACOT4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 17316-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of L02 using 17316-1-AP (same clone as 17316-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 17316-1-AP (ACOT4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17316-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 17316-1-AP (same clone as 17316-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17316-1-AP (ACOT4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17316-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 17316-1-AP (same clone as 17316-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17316-1-AP (ACOT4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17316-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 17316-1-AP (same clone as 17316-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17316-1-AP (ACOT4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17316-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 17316-1-AP (same clone as 17316-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ACOT4 antibody (17316-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17316-1-PBS in a different storage buffer formulation.

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Product Information

17316-1-PBS targets ACOT4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACOT4 fusion protein Ag11286
• Full Name: acyl-CoA thioesterase 4
• Calculated Molecular Weight: 421 aa, 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC031799
• Gene Symbol: ACOT4
• Gene ID (NCBI): 122970
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ACOT4, also known as PET2B and PTEIB, is an acyl-CoA thioesterases which catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), regulating their respective intracellular levels. ACOT4 has the strongest expression in liver and kidney and weaker expression in placenta, heart, and muscle(PMID: 16940157).