ACOT2 Polyclonal antibody

ACOT2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

7

Reviews (1)

Cat no : 15633-1-AP

Synonyms

EC:3.1.2.2, CTE-Ia, Acyl-coenzyme A thioesterase 2, mitochondrial, Acyl-coenzyme A thioester hydrolase 2a, Acyl-CoA thioesterase 2

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Validation Data Gallery

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  WB analysis using 15633-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15633-1-AP (ACOT2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 15633-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15633-1-AP (ACOT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15633-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15633-1-AP (ACOT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 15633-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15633-1-AP (ACOT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15633-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15633-1-AP (ACOT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15633-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15633-1-AP (ACOT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 15633-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 15633-1-AP (ACOT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 15633-1-AP

  IP result of anti-ACOT2 (IP:15633-1-AP, 3ug; Detection:15633-1-AP 1:500) with HepG2 cells lysate 1700ug.

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  IHC staining of human heart using 15633-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 15633-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 15633-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15633-1-AP (ACOT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 15633-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15633-1-AP (ACOT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skeletal muscle using 15633-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skeletal muscle using 15633-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 15633-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 15633-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 15633-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 15633-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 15633-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 15633-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 15633-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 15633-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 15633-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 15633-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 15633-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ACOT2 antibody (15633-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HepG2 using 15633-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ACOT2 antibody (15633-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse kidney tissue, HepG2 cells, human testis tissue, human brain tissue, human kidney tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human kidney tissue, human skeletal muscle tissue, human heart tissue, human testis tissue, human liver tissue, human spleen tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IF: See 1 publications below

Product Information

15633-1-AP targets ACOT2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACOT2 fusion protein Ag8093
• Full Name: acyl-CoA thioesterase 2
• Calculated Molecular Weight: 483 aa, 53 kDa
• Observed Molecular Weight: 46-53 kDa
• GenBank Accession Number: BC006335
• Gene Symbol: ACOT2
• Gene ID (NCBI): 10965
• RRID: AB_2221535
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Acyl-CoA thioesterase (Acot)2 localizes to the mitochondrial matrix and hydrolyses long-chain fatty acyl-CoA into free FA and CoASH. Acot2 is expressed in highly oxi­dative tissues and is poised to modulate mitochondrial FA oxidation (FAO) (PMID: 25114170). The structure of ACOT2 consists of two domains, N and C domains, and the active site of ACOT2 is located at the interface between the N and C domains (PMID: 19497300).

Publications

Species	Application	Title
mouse	WB	Eur J Immunol; YY1 control of mitochondrial-related genes does not account for regulation of immunoglobulin class switch recombination in mice.; Authors - Satabdi Nandi
mouse	WB	Life Sci Alliance; High levels of TFAM repress mammalian mitochondrial DNA transcription in vivo; Authors - Nina A Bonekamp
mouse	WB	Biomed Res Int; Up-Regulated MicroRNA-27b Promotes Adipocyte Differentiation via Induction of Acyl-CoA Thioesterase 2 Expression.; Authors - Yuka Murata
mouse	WB	J Biol Chem; Requirement of hepatic pyruvate carboxylase during fasting, high fat, and ketogenic diet; Authors - Ebru S. Selen
mouse	WB	EMBO Mol Med; A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy; Authors - Nneka Southwell
human	IF	Hepatol Commun; Dysregulation of lipid metabolism in the pseudolobule promotes region-specific autophagy in hepatitis B liver cirrhosis; Authors - Wenjun Pu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Lana (Verified Customer) (06-21-2019)

Lines on WB:1. BioRad Precision Plus Protein standards2. Homogenate of human cortex3. Non-synaptosomal mitochondria of human cortex4. Homogenate of mouse brains5. Non-synaptosomal mitochondria of mouse brains6. Whole cell lysate of rat PC12 cells7. Mitochondria of rat C12 cellsNotes: Specific band 48 kDa in human samples only. Strong non-specific band 40 kDa in all samples types.WB protocol:SDS-PAGE: 15 ug/ul of RIPA lysates, 4-12% Bis-tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore), O/N at 30V, 4C.Blocking: SEA Block Blocking Buffer 1hPrimary Ab: O/N incubation at 4C.Secondary Ab: IRDye 800CW Goat anti-Rabbit, 1 h incubation at room temperature.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Brain tissues, human and mouse, rat cell line.

ACOT2 Antibody Western Blot, validation (1:1000 dilution) in Brain tissues, human and mouse, rat cell line. (Cat no:15633-1-AP)