Aconitase 1 Polyclonal antibody

• Featured Product
• KD/KO Validated

Aconitase 1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 12406-1-AP

Synonyms

ACO1, ACO 1, EC:4.2.1.3, Citrate hydro-lyase, Aconitase1

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Validation Data Gallery

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  WB analysis using 12406-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12406-1-AP (Aconitase 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 12406-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12406-1-AP (Aconitase 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12406-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12406-1-AP (Aconitase 1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 12406-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12406-1-AP (Aconitase 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12406-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12406-1-AP (Aconitase 1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12406-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12406-1-AP (Aconitase 1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 12406-1-AP

  IP result of anti-Aconitase 1 (IP:12406-1-AP, 4ug; Detection:12406-1-AP 1:5000) with HepG2 cells lysate 1925 ug.

• 

  IP experiment of mouse liver using 12406-1-AP

  IP result of anti-Aconitase 1 (IP:12406-1-AP, 4ug; Detection:12406-1-AP 1:300) with mouse liver tissue lysate 2400 ug.

• 

  IP experiment of HepG2 using 12406-1-AP

  IP result of anti-Aconitase 1 (IP:12406-1-AP, 4ug; Detection:12406-1-AP 1:5000) with HepG2 cells lysate 1925 ug.

• 

  IF Staining of U2OS using 12406-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using 12406-1-AP (Aconitase 1 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse liver tissue, mouse kidney tissue, HepG2 cells, rat liver tissue
• Positive IP detected in: HepG2 cells, mouse liver tissue
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IP: See 1 publications below

Product Information

12406-1-AP targets Aconitase 1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Aconitase 1 fusion protein Ag3083
• Full Name: aconitase 1, soluble
• Calculated Molecular Weight: 889 aa, 98 kDa
• Observed Molecular Weight: 98 kDa
• GenBank Accession Number: BC018103
• Gene Symbol: Aconitase 1
• Gene ID (NCBI): 48
• RRID: AB_10642942
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mammalian cells possess two distinct isoforms of aconitase encoded by different genes: Aconitase 1 [ACO1; cytosolic aconitase (c-aconitase)] encoded by ACO1 and Aconitase 2 [ACO2; mitochondrial aconitase (m-aconitase)] encoded by ACO2. In addition to the enzymatic roles in the cytoplasm, ACO1 also is known to bind to mRNAs of specific genes and maintain iron homeostasis.

Publications

Species	Application	Title
human	WB	iScience; Asparagine starvation suppresses histone demethylation through iron depletion; Authors - Jie Jiang ; KD Validated
mouse	WB	Biochim Biophys Acta Mol Basis Dis; Iron deposition-induced ferroptosis in alveolar type II cells promotes the development of pulmonary fibrosis.; Authors - Haipeng Cheng
human,mouse	WB,IHC	Cell Biosci; Mutant huntingtin induces iron overload via up-regulating IRP1 in Huntington's disease.; Authors - Li Niu
human	WB	Drug Des Devel Ther; Curcumin induces apoptosis and protective autophagy in castration-resistant prostate cancer cells through iron chelation.; Authors - Chunguang Yang
human	WB	Biomolecules; Carbamoyl-Phosphate Synthase 1 as a Novel Target of Phomoxanthone A, a Bioactive Fungal Metabolite.; Authors - Sara Ceccacci
human	WB	JOJ Ophthalmol; Mitophagy, Ferritinophagy and Ferroptosis in Retinal Pigment Epithelial Cells Under High Glucose Conditions: Implications for Diabetic Retinopathy and Age-Related Retinal Diseases.; Authors - Lalit Pukhrambam Singh