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Aconitase 1 Polyclonal antibody
Aconitase 1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 12406-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, mouse liver tissue, mouse kidney tissue, HepG2 cells, rat liver tissue |
Positive IP detected in | HepG2 cells, mouse liver tissue |
Positive IF/ICC detected in | U2OS cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 10 publications below |
IHC | See 2 publications below |
IP | See 1 publications below |
Product Information
12406-1-AP targets Aconitase 1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Aconitase 1 fusion protein Ag3083 |
Full Name | aconitase 1, soluble |
Calculated Molecular Weight | 889 aa, 98 kDa |
Observed Molecular Weight | 98 kDa |
GenBank Accession Number | BC018103 |
Gene Symbol | Aconitase 1 |
Gene ID (NCBI) | 48 |
RRID | AB_10642942 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Mammalian cells possess two distinct isoforms of aconitase encoded by different genes: Aconitase 1 [ACO1; cytosolic aconitase (c-aconitase)] encoded by ACO1 and Aconitase 2 [ACO2; mitochondrial aconitase (m-aconitase)] encoded by ACO2. In addition to the enzymatic roles in the cytoplasm, ACO1 also is known to bind to mRNAs of specific genes and maintain iron homeostasis.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Aconitase 1 antibody 12406-1-AP | Download protocol |
IF protocol for Aconitase 1 antibody 12406-1-AP | Download protocol |
IP protocol for Aconitase 1 antibody 12406-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
iScience Asparagine starvation suppresses histone demethylation through iron depletion
| ||
Biochim Biophys Acta Mol Basis Dis Iron deposition-induced ferroptosis in alveolar type II cells promotes the development of pulmonary fibrosis. | ||
Cell Biosci Mutant huntingtin induces iron overload via up-regulating IRP1 in Huntington's disease. | ||
Drug Des Devel Ther Curcumin induces apoptosis and protective autophagy in castration-resistant prostate cancer cells through iron chelation. | ||
Biomolecules Carbamoyl-Phosphate Synthase 1 as a Novel Target of Phomoxanthone A, a Bioactive Fungal Metabolite. | ||
JOJ Ophthalmol Mitophagy, Ferritinophagy and Ferroptosis in Retinal Pigment Epithelial Cells Under High Glucose Conditions: Implications for Diabetic Retinopathy and Age-Related Retinal Diseases. |