ACC1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

ACC1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A11G10

Cat no : 67373-1-PBS

Synonyms

ACAC, ACACA, ACC, ACC1, ACCA, Acetyl CoA carboxylase 1

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Validation Data Gallery

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  WB analysis of HeLa using 67373-1-Ig (same clone as 67373-1-PBS)

  WB result of ACC1 antibody (67373-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACC1 transfected HeLa cells. This data was developed using the same antibody clone with 67373-1-PBS in a different storage buffer formulation.

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  WB analysis using 67373-1-Ig (same clone as 67373-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67373-1-Ig (ACC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67373-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67373-1-Ig (same clone as 67373-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67373-1-Ig (ACC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67373-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67373-1-Ig (same clone as 67373-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67373-1-Ig (ACC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67373-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67373-1-Ig (same clone as 67373-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67373-1-Ig (ACC1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67373-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67373-1-Ig (same clone as 67373-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ACC1 antibody (67373-1-Ig, Clone: 1A11G10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67373-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67373-1-PBS targets ACC1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ACC1 fusion protein Ag17503
• Full Name: acetyl-Coenzyme A carboxylase alpha
• Calculated Molecular Weight: 2383 aa, 275 kDa
• Observed Molecular Weight: 250-270 kDa
• GenBank Accession Number: BC137287
• Gene Symbol: ACACA
• Gene ID (NCBI): 31
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

ACACA(Acetyl-CoA carboxylase 1, ACC), also named as ACAC, ACC1 and ACCA, belongs to the biotin containing enzyme family. It catalyzes the synthesis of malonyl-CoA, which is an intermediate substrate playing a pivotal role in the regulation of fatty acid metabolism and energy production. ACACA is involved in the biosynthesis of fatty acids, and malonyl-CoA produced is used as a building block to extend the chain length of fatty acids by fatty acid synthase (FAS)(PMID:19900410). It has 4 isoforms produced by alternative promoter usage with the molecular weight between 260 kDa and 270 kDa.