ACBD5 Polyclonal antibody

ACBD5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 21080-1-AP

Synonyms

ACBD5, KIAA1996

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Validation Data Gallery

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  WB analysis using 21080-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21080-1-AP (ACBD5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 21080-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21080-1-AP (ACBD5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, mouse skeletal muscle tissue, L02 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

21080-1-AP targets ACBD5 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACBD5 fusion protein Ag15287
• Full Name: acyl-Coenzyme A binding domain containing 5
• Calculated Molecular Weight: 534 aa, 60 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC030555
• Gene Symbol: ACBD5
• Gene ID (NCBI): 91452
• RRID: AB_2878808
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACBD5, also known as RDLKD. It is expected to be located in the peroxisome membrane, which is ubiquitinated in brain and kidney tissue. This gene encodes a member of the acyl-Coenzyme A binding protein family, known to function in the transport and distribution of long chain acyl-Coenzyme A in cells. This gene may play a role in the differentiation of megakaryocytes and formation of platelets. A related protein in yeast is involved in autophagy of peroxisomes. A mutation in this gene has been associated with autosomal dominant thrombocytopenia. The molecular weight of ACBD5 is 60 kDa.

Publications

Species	Application	Title
mouse	WB	J Biol Chem; Long-chain acyl-CoA synthetase 1 interacts with key proteins that activate and direct fatty acids into niche hepatic pathways.; Authors - Pamela A Young
mouse	WB	Mol Cell Biochem; Differential distribution of peroxisomal proteins points to specific roles of peroxisomes in the murine retina.; Authors - Yannick Das