ACAT2 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

ACAT2 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F5F11

Cat no : 68005-1-PBS

Synonyms

ACAT2, ACTL

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Validation Data Gallery

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  WB analysis using 68005-1-Ig (same clone as 68005-1-PBS)

  Various cell lysates were subjected to SDS PAGE followed by western blot with 68005-1-Ig (ACAT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68005-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 68005-1-Ig (same clone as 68005-1-PBS)

  WB result of ACAT2 antibody (68005-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAT2 transfected HepG2 cells. This data was developed using the same antibody clone with 68005-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68005-1-Ig (same clone as 68005-1-PBS)

  Brain tissues were subjected to SDS PAGE followed by western blot with 68005-1-Ig (ACAT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68005-1-PBS in a different storage buffer formulation.

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  WB analysis of HCT 116 using 68005-1-Ig (same clone as 68005-1-PBS)

  HCT 116 cells were subjected to SDS PAGE followed by western blot with 68005-1-Ig (ACAT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68005-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 68005-1-Ig (same clone as 68005-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68005-1-Ig (ACAT2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68005-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68005-1-Ig (same clone as 68005-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ACAT2 antibody (68005-1-Ig, Clone: 1F5F11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68005-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68005-1-PBS targets ACAT2 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.

• Tested Reactivity: Human, mouse, rat, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ACAT2 fusion protein Ag6596
• Full Name: acetyl-Coenzyme A acetyltransferase 2
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC000408
• Gene Symbol: ACAT2
• Gene ID (NCBI): 39
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.