ACAP3 Polyclonal antibody

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  WB analysis of Jurkat using 17570-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17570-1-AP (ACAP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 17570-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17570-1-AP (ACAP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of SH-SY5Y using 17570-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed SH-SY5Y cells using 17570-1-AP (ACAP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

ACAP3 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 17570-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

KIAA1716, Cnt-b5, CENTB5, Centaurin-beta-5, Centaurin beta 5

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Tested Applications

• Positive WB detected in: Jurkat cells, SH-SY5Y cells
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Product Information

17570-1-AP targets ACAP3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACAP3 fusion protein Ag11777
• Full Name: ArfGAP with coiled-coil, ankyrin repeat and PH domains 3
• Calculated Molecular Weight: 834 aa, 92 kDa
• Observed Molecular Weight: 85kda, 92 kDa
• GenBank Accession Number: BC047001
• Gene Symbol: ACAP3
• Gene ID (NCBI): 116983
• RRID: AB_2257668
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96P50
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACAP3 (Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 3) , also known as CENTB5, is a protein-coding gene that belongs to the ACAP family of GTPase-activating proteins (GAPs). ACAP3 is involved in neuronal migration in the developing cerebral cortex of mice. Knockdown of ACAP3 in the developing cortical neurons of mice in utero significantly abrogated neuronal migration in the cortical layer, ACAP3 is involved in neuronal migration in the developing cerebral cortex of mice. Knockdown of ACAP3 in the developing cortical neurons of mice in utero significantly abrogated neuronal migration in the cortical layer (PMID: 28919417),

Publications

Species	Application	Title
human,mouse	WB,IF	Biochem J; ACAP3 regulates neurite outgrowth through its GAP activity specific to Arf6 in mouse hippocampal neurons.; Authors - Yuki Miura
mouse	WB	Biochem Biophys Res Commun; ACAP3, the GTPase-activating protein specific to the small GTPase Arf6, regulates neuronal migration in the developing cerebral cortex.; Authors - Yuki Miura ; KD Validated
mouse	WB	Autophagy; Aging Differentially Affects Axonal Autophagosome Formation and Maturation; Authors - Heather Tsong