ACAP3 Polyclonal antibody

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  WB analysis of Jurkat using 17570-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17570-1-AP (ACAP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 17570-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17570-1-AP (ACAP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of SH-SY5Y using 17570-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed SH-SY5Y cells using 17570-1-AP (ACAP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

ACAP3 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 17570-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

KIAA1716, Cnt-b5, CENTB5, Centaurin-beta-5, Centaurin beta 5

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Tested Applications

• Positive WB detected in: Jurkat cells, SH-SY5Y cells
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Product Information

17570-1-AP targets ACAP3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACAP3 fusion protein Ag11777
• Full Name: ArfGAP with coiled-coil, ankyrin repeat and PH domains 3
• Calculated Molecular Weight: 834 aa, 92 kDa
• Observed Molecular Weight: 92 kDa
• GenBank Accession Number: BC047001
• Gene Symbol: ACAP3
• Gene ID (NCBI): 116983
• RRID: AB_2257668
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96P50
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB,IF	Biochem J; ACAP3 regulates neurite outgrowth through its GAP activity specific to Arf6 in mouse hippocampal neurons.; Authors - Yuki Miura
mouse	WB	Biochem Biophys Res Commun; ACAP3, the GTPase-activating protein specific to the small GTPase Arf6, regulates neuronal migration in the developing cerebral cortex.; Authors - Yuki Miura ; KD Validated
mouse	WB	Autophagy; Aging Differentially Affects Axonal Autophagosome Formation and Maturation; Authors - Heather Tsong