ACAP1 Recombinant antibody

• Featured Product
• KD/KO Validated

ACAP1 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230370D5

Cat no : 82967-4-RR

Synonyms

Cnt b1, CENTB1, Centaurin-beta-1, Centaurin beta 1, Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1

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Validation Data Gallery

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  WB analysis of Jurkat using 82967-4-RR

  WB result of ACAP1 antibody (82967-4-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAP1 transfected Jurkat cells.

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  WB analysis using 82967-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82967-4-RR (ACAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of Jurkat using 82967-4-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ACAP1 antibody (82967-4-RR, Clone: 230370D5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of A431 using 82967-4-RR

  1x10^6 A431 cells were intracellularly stained with 0.4 ug ACAP1 Recombinant antibody (82967-4-RR, Clone:230370D5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82967-4-RR

  Biolayer interferometry (BLl) kinetic assays of 82967-4-RR against Human ACAP1 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IF/ICC detected in: Jurkat cells
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82967-4-RR targets ACAP1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ACAP1 fusion protein Ag34309
• Full Name: ArfGAP with coiled-coil, ankyrin repeat and PH domains 1
• Calculated Molecular Weight: 740 aa, 82 kDa
• Observed Molecular Weight: 82-85 kDa
• GenBank Accession Number: BC018543
• Gene Symbol: ACAP1
• Gene ID (NCBI): 9744
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACAP1, also named as Centaurin-beta-1, is a 740 amino acid protein, which is expressed Highly in lung and spleen. ACAP1 as a GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) is required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface and required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.