ACADM Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

ACADM Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 55210-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 55210-1-AP (same clone as 55210-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55210-1-AP (ACADM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55210-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 55210-1-AP (same clone as 55210-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55210-1-AP (ACADM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55210-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat heart using 55210-1-AP (same clone as 55210-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 55210-1-AP (ACADM Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55210-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 55210-1-AP (same clone as 55210-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 55210-1-AP (ACADM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55210-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 55210-1-AP (same clone as 55210-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55210-1-AP (ACADM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55210-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 55210-1-AP (same clone as 55210-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55210-1-AP (ACADM antibody) at dilution of 1:150 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55210-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 55210-1-AP (same clone as 55210-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55210-1-AP (ACADM antibody) at dilution of 1:150 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55210-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 55210-1-AP (same clone as 55210-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 55210-1-AP (ACADM antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55210-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 55210-1-AP (same clone as 55210-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 55210-1-AP (ACADM antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55210-1-PBS in a different storage buffer formulation.

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Product Information

55210-1-PBS targets ACADM in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: NM_000016
• Gene Symbol: ACADM
• Gene ID (NCBI): 34
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ACADM, also named as MCAD, belongs to the acyl-CoA dehydrogenase family. This enzyme is specific for acyl chain lengths of 4 to 16. It catalyzes the reaction: Acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor. Defects in ACADM are the cause of medium-chain acyl-CoA dehydrogenase deficiency (MCAD deficiency). This protein can exsit as a dimer(PMID:8962055). This antibody is specific to ACADM.