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ACADL-Specific Polyclonal antibody
ACADL-Specific Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (4)
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 17526-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse kidney tissue, mouse heart tissue, mouse liver tissue, mouse lung tissue |
Positive IP detected in | mouse kidney tissue |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 3 publications below |
WB | See 45 publications below |
IHC | See 5 publications below |
IF | See 2 publications below |
IP | See 4 publications below |
Product Information
17526-1-AP targets ACADL-Specific in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat, pig, sheep, ewes |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | acyl-Coenzyme A dehydrogenase, long chain |
Calculated Molecular Weight | 48 kDa |
Observed Molecular Weight | 48 kDa |
GenBank Accession Number | NM_001608 |
Gene Symbol | ACADL |
Gene ID (NCBI) | 33 |
RRID | AB_2219661 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ACADL(Long-chain specific acyl-CoA dehydrogenase, mitochondrial) is also named as LCAD and belongs to the acyl-CoA dehydrogenase family.It catalyzes the first reaction of the mitochondrial β-oxidation of fatty acids and is synthesized in the cytosol as a precursor that is larger than its mature from. The size difference between the precursor and mature rat LCAD has been estimated to be 3 kDa on the basis of SDS-PAGE(PMID:1774065).Defects in ACADL are a cause of acyl-CoA dehydrogenase very long-chain deficiency (ACADVLD).This antibody is specific to ACADL.
Protocols
Product Specific Protocols | |
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WB protocol for ACADL-Specific antibody 17526-1-AP | Download protocol |
IP protocol for ACADL-Specific antibody 17526-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Res Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation | ||
Int J Biol Sci Bulk and single-cell transcriptome profiling reveal extracellular matrix mechanical regulation of lipid metabolism reprograming through YAP/TEAD4/ACADL axis in hepatocellular carcinoma | ||
Diabetes Pivotal Role of O-GlcNAc Modification in Cold-Induced Thermogenesis by Brown Adipose Tissue Through Mitochondrial Biogenesis. | ||
Phytomedicine Shen-Shuai-II-Recipe inhibits tubular inflammation by PPARα-mediated fatty acid oxidation to attenuate fibroblast activation in fibrotic kidneys | ||
Cell Rep HIF-1-Mediated Suppression of Acyl-CoA Dehydrogenases and Fatty Acid Oxidation Is Critical for Cancer Progression.
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Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Rebeca (Verified Customer) (03-11-2024) | I had a great experience ordering this product and it arrived within a few days.
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FH Wu (Verified Customer) (04-10-2021) | LCAD antibody worked good in IF assay at 1:50 dilution.
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FH Tanusree (Verified Customer) (12-18-2019) | Product worked well in WB at 1:500 dilution
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