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  • KD/KO Validated

ACADL-Specific Polyclonal antibody

ACADL-Specific Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 17526-1-AP

Synonyms

ACAD4, ACADL, ACADL-Specific, LCAD



Tested Applications

Positive WB detected inmouse kidney tissue, mouse heart tissue, mouse liver tissue, mouse lung tissue
Positive IP detected inmouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17526-1-AP targets ACADL-Specific in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, pig, sheep, ewes
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name acyl-Coenzyme A dehydrogenase, long chain
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberNM_001608
Gene Symbol ACADL
Gene ID (NCBI) 33
RRIDAB_2219661
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACADL(Long-chain specific acyl-CoA dehydrogenase, mitochondrial) is also named as LCAD and belongs to the acyl-CoA dehydrogenase family.It catalyzes the first reaction of the mitochondrial β-oxidation of fatty acids and is synthesized in the cytosol as a precursor that is larger than its mature from. The size difference between the precursor and mature rat LCAD has been estimated to be 3 kDa on the basis of SDS-PAGE(PMID:1774065).Defects in ACADL are a cause of acyl-CoA dehydrogenase very long-chain deficiency (ACADVLD).This antibody is specific to ACADL.

Protocols

Product Specific Protocols
WB protocol for ACADL-Specific antibody 17526-1-APDownload protocol
IP protocol for ACADL-Specific antibody 17526-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Res

Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation

Authors - Yunzi Mao
mouseWB

Circ Res

NAD+ Repletion Reverses Heart Failure With Preserved Ejection Fraction.

Authors - Dan Tong
humanWB,IF

Int J Biol Sci

Bulk and single-cell transcriptome profiling reveal extracellular matrix mechanical regulation of lipid metabolism reprograming through YAP/TEAD4/ACADL axis in hepatocellular carcinoma

Authors - Jingwei Cai
mouseWB

Diabetes

Pivotal Role of O-GlcNAc Modification in Cold-Induced Thermogenesis by Brown Adipose Tissue Through Mitochondrial Biogenesis.

Authors - Natsuko Ohashi
ratWB

Phytomedicine

Shen-Shuai-II-Recipe inhibits tubular inflammation by PPARα-mediated fatty acid oxidation to attenuate fibroblast activation in fibrotic kidneys

Authors - Meng Wang
mouse, humanWB, IHC

Cell Rep

HIF-1-Mediated Suppression of Acyl-CoA Dehydrogenases and Fatty Acid Oxidation Is Critical for Cancer Progression.

Authors - De Huang
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Rebeca (Verified Customer) (03-11-2024)

I had a great experience ordering this product and it arrived within a few days.

  • Applications: Western Blot
FH

Wu (Verified Customer) (04-10-2021)

LCAD antibody worked good in IF assay at 1:50 dilution.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Liver
ACADL-Specific Antibody Immunofluorescence validation (1:50 dilution) in Liver (Cat no:17526-1-AP)
FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse brain