STARS/ABRA Polyclonal antibody

STARS/ABRA Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 22673-1-AP

Synonyms

ABRA, Striated muscle activator of Rho-dependent signaling, STARS, Actin-binding Rho-activating protein

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Validation Data Gallery

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  WB analysis of mouse heart using 22673-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 22673-1-AP (STARS/ABRA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 22673-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 22673-1-AP (STARS/ABRA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 22673-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 22673-1-AP (STARS/ABRA antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 22673-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 22673-1-AP (STARS/ABRA antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 22673-1-AP

  Immunofluorescent analysis of HepG2 cells using 22673-1-AP (STARS/ABRA antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse heart tissue, human heart tissue
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 2 publications below

Product Information

22673-1-AP targets STARS/ABRA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STARS/ABRA fusion protein Ag18456
• Full Name: actin-binding Rho activating protein
• Calculated Molecular Weight: 381 aa, 43 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC105103
• Gene Symbol: STARS/ABRA
• Gene ID (NCBI): 137735
• RRID: AB_11182712
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IF	Development; HAND2-mediated epithelial maintenance and integrity in cardiac outflow tract morphogenesis.; Authors - Meng Xia
mouse	WB	Behav Brain Res; Changes in mRNA and miRNA expression in the prelimbic cortex related to depression-like syndrome induced by chronic social defeat stress in mice; Authors - Yexiang Chen
human,mouse	WB,IF	Am J Pathol; Different mouse models of nemaline myopathy harboring Acta1 mutations display differing abnormalities related to mitochondrial biology; Authors - Jennifer A Tinklenberg