ABL1 Monoclonal antibody

ABL1 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1E7A5

Cat no : 68254-1-Ig

Synonyms

c ABL, JTK7, EC:2.7.10.2, ABL, Abelson tyrosine-protein kinase 1

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Validation Data Gallery

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  WB analysis using 68254-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68254-1-Ig (ABL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 68254-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using ABL1 antibody (68254-1-Ig, Clone: 1E7A5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of U2OS using 68254-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ABL1 antibody (68254-1-Ig, Clone: 1E7A5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of MCF-7 using 68254-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ABL1 antibody (68254-1-Ig, Clone: 1E7A5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  FC experiment of K-562 using 68254-1-Ig

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human ABL1 (68254-1-Ig, Clone:1E7A5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
• Positive IF/ICC detected in: U2OS cells, HepG2 cells, MCF-7 cells
• Positive FC (Intra) detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68254-1-Ig targets ABL1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: ABL1 fusion protein Ag26152
• Full Name: c-abl oncogene 1, receptor tyrosine kinase
• Calculated Molecular Weight: 123 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: NM_005157
• Gene Symbol: ABL1
• Gene ID (NCBI): 25
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ABL1, also named as ABL, JTK7, c-ABL and p150, belongs to the protein kinase superfamily, Tyr protein kinase family and ABL subfamily. ABL1 regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. ABL1 localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. It regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired. ABL1 catalyze the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. A chromosomal aberration involving ABL1 is a cause of chronic myeloid leukemia (CML) [MIM:608232]. Translocation t(9;22)(q34;q11) with BCR. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL). The antibody is specific to ABL1.