ABHD4 Polyclonal antibody

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  WB analysis using 32036-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32036-1-AP (ABHD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ABHD4 Polyclonal Antibody for WB, ELISA

Cat No. 32036-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Alpha/beta-hydrolase 4, Alpha/beta hydrolase domain-containing protein 4, Abhydrolase domain-containing protein 4, Abhydrolase Domain Containing 4, N-Acyl Phospholipase B, Abhydrolase Domain Containing 4

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Tested Applications

• Positive WB detected in: A549 cells, HepG2 cells, LNCap cells, SiHa cells, mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32036-1-AP targets ABHD4 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ABHD4 fusion protein Ag36373
• Full Name: abhydrolase domain containing 4
• Calculated Molecular Weight: 342 aa, 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC024779
• Gene Symbol: ABHD4
• Gene ID (NCBI): 63874
• RRID: AB_3670176
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q8TB40
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The alpha/beta hydrolase structural domain-containing protein 4 (ABHD4) gene is a major regulator of mammalian phospholipid metabolism with hydrolase and lysophospholipase activities. ABHD4 is involved in anoikis resistance and endogenous cannabinoid biosynthesis, and ABHD4-mediated developmental anoikis specifically protects embryonic brains from the consequences of sporadic delamination errors and teratogenic damage.