ABHD11 Polyclonal antibody

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  WB analysis using 25355-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25355-1-AP (ABHD11 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-3 using 25355-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using ABHD11 antibody (25355-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

ABHD11 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 25355-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

sn-1-specific diacylglycerol lipase ABHD11, PP1226, EC:3.1.1.116, Alpha/beta hydrolase domain-containing protein 11, Abhydrolase domain-containing protein 11

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Tested Applications

• Positive WB detected in: MCF-7 cells, PC-3 cells, human placenta tissue
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25355-1-AP targets ABHD11 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ABHD11 fusion protein Ag18318
• Full Name: abhydrolase domain containing 11
• Calculated Molecular Weight: 315 aa, 35 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC067750
• Gene Symbol: ABHD11
• Gene ID (NCBI): 83451
• RRID: AB_3669471
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NFV4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Abhydrolase domain-containing protein 11 (ABHD11) is a serine hydrolase enzyme, localized in mitochondria, that can synthesize the endocannabinoid 2-arachidonoyl glycerol (2AG) in vitro.