Validation Data Gallery
|Positive WB detected in||HUVEC cells, RAW 264.7 cells, HeLa cells, HepG2 cells, mouse kidney tissue, rat kidney tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||mouse placenta tissue, human tonsillitis tissue, human cervical cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
13578-1-AP targets ABCG1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ABCG1 fusion protein Ag4479|
|Full Name||ATP-binding cassette, sub-family G (WHITE), member 1|
|Calculated molecular weight||678 aa, 76 kDa|
|Observed molecular weight||75 kDa|
|GenBank accession number||BC029158|
|Gene ID (NCBI)||9619|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
ABCG1 is a cholesterol and phospholipid transporter expressed in lung, brain, spleen, and macrophages. In liver, ABCG1 is expressed mainly in Kupffer cells. The monomer of ABCG1 is around 60-65 kDa, while multiple forms of ABCG1 can be observed on SDS-PAGE, including 130 kDa (dimer), 90-95 kDa, 110 kDa. It was not clear whether the glycosylation or splicing accounts for the differences in mass. (PMID: 16702602)
Metabolic effects of RUBCN/Rubicon deficiency in kidney proximal tubular epithelial cells.
Br J Pharmacol
Functional interplay between LXR and AMPKα inhibits atherosclerosis in apoE deficient mice - A new anti-atherogenic strategy.
J Control Release
Artemisinin and Procyanidins loaded multifunctional nanocomplexes alleviate atherosclerosis via simultaneously modulating lipid influx and cholesterol efflux.
Br J Pharmacol
Targeting macrophage liver X receptors by hydrogel-encapsulated T0901317 reduces atherosclerosis without effect on hepatic lipogenesis.
Cyclodextrin boostered-high density lipoprotein for antiatherosclerosis by regulating cholesterol efflux and efferocytosis.
Int J Mol Sci
Exercise Ameliorates Atherosclerosis via Up-Regulating Serum β-Hydroxybutyrate Levels.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Shiford (Verified Customer) (09-24-2023)
We sorted cancer stem cell subpopulations from multiple cell lines by flow cytometry and used a cluster of antibodies related to stem cell characteristics to detect. ABCG1 is one of the possible stem cell markers, with clear bands.